Endogenous Nampt upregulation is associated with diabetic nephropathy inflammatory-fibrosis through the NF-κB p65 and Sirt1 pathway; NMN alleviates diabetic nephropathy inflammatory-fibrosis by inhibiting endogenous Nampt

Experimental and Therapeutic Medicine
Ye ChenLeping Feng

Abstract

Nicotinamide phosphoribosyltransferase (Nampt) is a key enzyme in the nicotinamide adenine dinucleotide (NAD(+)) biosynthetic pathway. Exogenous extra cellular Nampt has been reported to increase the synthesis of pro-fibrotic molecules in various types of renal cells. However, the role of endogenous Namptenzymatic activity in diabetic renal cells, particularly those associated with inflammation and fibrosis through the nuclear factor (NF)-κB p65 and sirtuin 1 (Sirt1) pathway is still unknown. In the present study, a possible mechanism by which endogenous Nampt upregulation affects the expression of pro-inflammatory and pro-fibrotic cytokines in vivo and in vitro, is reported. The present results demonstrate that the expression of vimentin and fibronectin was directly implicated in endogenous Nampt upregulation. The expression levels of Poly(ADP-ribose) polymerase-1, NF-κB p65, forkhead box protein O1 and B-cell lymphoma 2-like protein 4 were also significantly increased at 96 h compared with control group (P<0.01) respectively in response to endogenous Nampt upregulation. Furthermore, the expression level of Sirt1 was significantly reduced (P<0.05), and the NAD and NADH levels, and the NAD/NADH ratio are significantly altered i...Continue Reading

References

May 29, 2000·Molecular Human Reproduction·R KonnoA Yajima
Dec 18, 2004·Science·Atsunori FukuharaIichiro Shimomura
Sep 15, 2007·Proceedings of the National Academy of Sciences of the United States of America·Julie StøyUNKNOWN Neonatal Diabetes International Collaborative Group
Sep 5, 2008·American Journal of Physiology. Renal Physiology·Hye Kyoung SongDae Ryong Cha
Mar 17, 2009·Science·Yasukazu NakahataPaolo Sassone-Corsi
Apr 14, 2010·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Samuel CaitoIrfan Rahman
Mar 17, 2012·Clinical Biochemistry·Hala O El MesallamyAmira S Ahmed
Apr 3, 2012·Annual Review of Nutrition·Tuva B DahlBente Halvorsen
Jun 13, 2012·The Journal of Biological Chemistry·Franziska BuschMehdi Shakibaei
Mar 29, 2013·PloS One·Karen KoverWayne V Moore
Sep 17, 2013·PloS One·Yusuke S HoriYoshiyuki Horio
Nov 30, 2013·Biochimica Et Biophysica Acta·Alberto Benito-MartinAlberto Ortiz
Mar 19, 2015·Journal of Diabetes Research·Javier Donate-CorreaJuan F Navarro-González
Jun 18, 2015·Kidney International·Shu WakinoHiroshi Itoh
Jul 29, 2015·Nature Reviews. Endocrinology·Antje GartenWieland Kiess
Mar 31, 2016·Current Drug Metabolism·Riccardo PofiBiagio Barbano
Jun 17, 2016·Molecular & Cellular Oncology·Tom Vanden BerghePeter Vandenabeele
Feb 20, 2017·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Qinqin LinZhenjun Tian

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Citations

May 16, 2020·Frontiers in Cell and Developmental Biology·Weiqi HongXiawei Wei
Aug 10, 2021·Frontiers in Medicine·Jing XuDaisuke Koya

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Methods Mentioned

BETA
PCR
electrophoresis
fluorescence microscopy

Software Mentioned

SPSS
Pro Plus
Image J
Gene Tools
Image
VisionWorksLS

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