Endogenous nitric oxide inhibits leukotriene B4 release from rat alveolar macrophages

European Journal of Pharmacology
G BrunnK Racké

Abstract

Effects of endogenous nitric oxide (NO) on the release of mediators of the lipoxygenase and cyclo-oxygenase pathway from rat alveolar macrophages were studied. Alveolar macrophages, freshly isolated or after 18-h culture, were incubated in (amino acid-free) Krebs medium and labelled with [3H]arachidonic acid. The release of [3H]leukotriene B4 and [3H]prostanoids (separated by high performance liquid chromatography) was determined. A 23187 was used as stimulus, as rising intracellular Ca2+ activates directly the phospholipase A2 and lipoxygenase pathway. A 23187 (10 microM) enhanced [3H]leukotriene B4 release from freshly prepared alveolar macrophages about 65-fold, but only 5- to 6-fold from cultured alveolar macrophages. Evoked [3H]leukotriene B4 release and spontaneous [3H]prostanoid release were inhibited when L-arginine (300 microM) was added to the Krebs incubation medium of alveolar macrophages, in which marked NO synthase had been induced by culture with lipopolysaccharides (10 microg/ml). Inhibitory effects of L-arginine were prevented by N(G)-monomethyl-L-arginine (L-NMMA, 100 microM). Inhibition of NO synthase during the culture period by L-NMMA (culture medium, in contrast to Krebs medium, already contains the substr...Continue Reading

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Citations

Jun 27, 2003·European Journal of Pharmacology·Sahra KoschorreckKurt Racké
Aug 5, 2003·Prostaglandins, Leukotrienes, and Essential Fatty Acids·M Peters-Golden, T G Brock
Nov 1, 2001·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·M S Dykewicz
Jul 29, 1999·American Journal of Respiratory Cell and Molecular Biology·S P Sanders
Oct 11, 2005·Journal of Cellular Biochemistry·Thomas G Brock
Oct 18, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·M J CoffeyM Peters-Golden
Jan 7, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Marc Peters-GoldenMichael J Coffey
Apr 6, 2017·Frontiers in Pharmacology·Nadeem BilaniWassim Abou-Kheir
Aug 23, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Shi Yan LeeKian-Kai Cheng
Jun 30, 2000·Pulmonary Pharmacology & Therapeutics·R HammermannK Racké
Apr 13, 2000·Biochimica Et Biophysica Acta·L CaloriniS Ruggieri

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