Endogenous nucleosides in the guinea-pig eye: analysis of transport and metabolites

Experimental Eye Research
Z B RedzićL M Rakic

Abstract

This study investigates the transport of endogenous nucleosides and deoxynucleosides from the capillaries of the eye into the aqueous humour and the lens using the in situ vascular eye perfusion technique in the guinea-pig. The transport of [3H] adenosine and [3H] thymidine across the blood-aqueous barrier proved to be very rapid with a volume of distribution after 4 minutes perfusion reaching 11.9+/-3.0% and 9.93+/-1.1%, respectively. However, the transport of [3H] guanosine and [3H] cytidine was slower, with volumes of distribution reaching only 3.38+/-0.58% and 4.8+/-1.41%. The values for the entry of deoxyadenosine and deoxyguanosine were not significantly different from the values obtained for corresponding ribonucleosides (adenosine and guanosine) so that a change in the pentose sugar does not change the affinity of the nucleoside for the transport protein. Perfusion with a low sodium medium inhibited the transport of [3H] adenosine and [3H] thymidine into the aqueous humour. The presence of 800 nM NBTI also caused a decrease in adenosine transport into the aqueous humour, so that the volume of distribution after 2 minutes reached only 3.78+/-1.87%. These findings suggest that the transfer of adenosine across the blood-aq...Continue Reading

References

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Citations

Oct 13, 2007·Progress in Retinal and Eye Research·Ana Guzmán-AranguezJesus Pintor
Apr 8, 2014·Experimental Eye Research·Martin KronschlägerPer Söderberg
Jan 13, 2005·Canadian Journal of Physiology and Pharmacology·D DekanskiD M Mitrović
Jun 14, 2000·Progress in Neurobiology·M P RathboneF Caciagli
Jan 17, 2002·Brain Research·Zoran B RedzicLjubisa M Rakic

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