Apr 14, 1976

Endogenous proteolytic activity and constituent polypeptide chains of sheep and pig 19 S thyroglobulin

Biochimica Et Biophysica Acta
M Rolland, S Lissitzky

Abstract

Porcine and ovine 19-S thyroglobulins prepared from frozen glands in several buffers using slice extraction or homogenization, ammonium sulfate precipitation and DEAE-cellulose chromatography or Sepharose 6B gel filtration were contaminated with protease activity of pH optima 4.5 and 8.6, as shown by sodium dodecyl sulfate polyacrylamide gel electrophoresis. Optimum temperatures of autodigestion were 37 degrees C at pH 4.5 and 25 degrees C at pH 8.6. Thyroglobulins prepared from unfrozen glands pH 7.2 in 0.1 M sodium phosphate using slice extraction, ammonium sulfate precipitation and Sepharose 6B gel filtration were devoid of acid proteolytic activity but still underwent autodigestion at pH 8.6. Diisopropylfluorophosphate was a potent inhibitor of the alkaline protease activity of ovine thyroglobulin preparations. In contrast to thyroglobulin obtained from frozen glands the proteins purified from fresh unfrozen glands at pH 7.2 only showed the 19-S and the 12-S species by electrophoresis in sodium dodecyl sulfate polyacrylamide gels. Very few bands migrating faster than 12-S were visible. After full reduction and S-alkylation of porcine and ovine thyroglobulins, no qualitative changes were observed in the gel electrophoresis p...Continue Reading

  • References4
  • Citations23

References

Mentioned in this Paper

Buffers
Macromolecular Compounds
Recombinant Thyroglobulin
Polyacrylamide Gels
Isoflurophate
SDS-PAGE
Peptide Hydrolases
Chromatography, DEAE-Cellulose
Dall Sheep
Iodine

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