Endogenous reverse transcriptase: a mediator of cell proliferation and differentiation

Cytogenetic and Genome Research
Corrado Spadafora

Abstract

Endogenous, non-telomeric Reverse Transcriptase (RT) is encoded by two classes of repeated genomic elements, retrotransposons and endogenous retroviruses, and is an essential component of the retrotransposition machinery of both types of elements. Expression of RT-coding genes is generally repressed in non-pathological, terminally differentiated cells, but is active in early embryos, germ cells, embryo and tumor tissues, all of which have a high proliferative potential. To clarify whether reverse transcription is functionally implicated in control of cell growth, differentiation and in embryogenesis, recent experiments have been undertaken to inactivate the endogenous RT activity. RT was inhibited in normal and transformed cell lines by exposure to nevirapine, a non-nucleosidic RT inhibitor. The endogenous RT was also blocked in murine embryos by microinjection of an anti-RT antibody. Both experimental approaches yielded a dramatic inhibition of proliferation. Murine embryos arrested at pre-implantation stages. Transformed cell lines underwent a significant reduction in the rate of cell growth, concomitant with the induction of differentiation. In addition, RT inhibition induced an extensive reprogramming of the gene expression...Continue Reading

Citations

Aug 7, 2013·Advances in Biological Regulation·Siti Nornadhirah AmdaniKevin Coward
Jan 17, 2009·Human Molecular Genetics·Ioannis GeorgiouTheodore Tzavaras
Jun 25, 2010·Human Reproduction Update·J KashirK Coward
Jul 9, 2008·Biochemistry. Biokhimii︠a︡·S S Ryazansky, V A Gvozdev
Dec 6, 2007·Genome Biology·Lara S Collier, David A Largaespada
Apr 16, 2005·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Kevin Smith, Corrado Spadafora
Jul 2, 2009·Xenotransplantation·Jagdeece RamsoondarDavid Ayares
Mar 21, 2008·International Journal of Cancer. Journal International Du Cancer·Matteo LandriscinaElena Ranieri
Dec 21, 2005·Molecular Reproduction and Development·Rosanna BeraldiCorrado Spadafora
Mar 9, 2011·Birth Defects Research. Part A, Clinical and Molecular Teratology·Kenneth S RamosVilius Stribinskis
Mar 25, 2014·Reproductive Biomedicine Online·Frauke Vanden MeerschautPetra De Sutter
Sep 30, 2008·Gynecologic Oncology·Konstantinos StefanidisAris Antsaklis
May 1, 2012·Free Radical Biology & Medicine·Sofia KonistiTheodore Tzavaras
Dec 3, 2013·Cell Calcium·Queenie V NeriGianpiero D Palermo
May 22, 2007·Biochemical Society Transactions·R C Crowell, A A Kiessling
Sep 19, 2020·Nature Communications·Michelle M HalsteadPablo J Ross

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