PMID: 3768394Nov 14, 1986Paper

Endogenous suppression of neutral-active and calcium-dependent phospholipase A2 in human polymorphonuclear leukocytes

Biochimica Et Biophysica Acta
F Märki, R C Franson

Abstract

Phospholipase A2 activity was measured in homogenized and acid-extracted human polymorphonuclear leukocytes using [1-14C]oleate-labelled autoclaved Escherichia coli as substrate. In whole homogenate and in the supernatant and particular fractions separated by centrifugation at 150,000 X g, phospholipase activity was barely detectable (1-4 pmol/h per 10(6) cell equivalents). By contrast, acid extracts of these fractions contained over 10-times as much phospholipase activity in the dialyzed supernatants (20-300 pmol/h per 10(6) cell equivalents), whereas phospholipase inhibitor(s) were found in the sediment. The acid-solubilized phospholipase A2 activity was absolutely Ca2+-dependent and optimal at pH 7.0-7.5 with 1.0 mM added Ca2+. Addition of the resuspended sediment of the acid extract dose-dependently suppressed phospholipase activity in the supernatant; less than equivalent amounts were sufficient to inhibit 95%. Suppressor activity was lipid-extractable. After thin layer chromatography of lipid extracts, the bulk of inhibitory activity was recovered from the free fatty acid region. Analysis of the fatty acids by gas liquid chromatography showed that 63% were unsaturated. All unsaturated fatty acids tested were potent inhibi...Continue Reading

References

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Citations

Jan 1, 1993·European Journal of Clinical Pharmacology·J PfeilschifterH van den Bosch
Jun 1, 1989·Agents and Actions·W GalbraithJ S Kerr
Mar 1, 1993·Agents and Actions·F MärkiR Wiederkehr
Nov 1, 1994·Agents and Actions·C Rordorf-AdamK Vosbeck
Dec 5, 2012·International Journal of Legal Medicine·Andrea FallerGisela Skopp
May 1, 1990·Biochimica Et Biophysica Acta·C G SchalkwijkH Van den Bosch
Nov 3, 1992·Biochemical Pharmacology·L A Marshall, A McCarte-Roshak
May 29, 2004·Biochimica Et Biophysica Acta·Claudia PetryAndrea Huwiler
Jun 16, 1999·British Journal of Pharmacology·G RupprechtM Kaszkin
Oct 1, 1993·The Journal of Clinical Investigation·B S VishwanathB M Frey
Jul 15, 1996·The Journal of Clinical Investigation·B S VishwanathB M Frey
Feb 1, 1992·Scandinavian Journal of Clinical and Laboratory Investigation·N E SarisG E Hoffmann
Aug 4, 1999·Critical Reviews in Clinical Laboratory Sciences·E Kaiser
Nov 1, 1993·European Journal of Clinical Chemistry and Clinical Biochemistry : Journal of the Forum of European Clinical Chemistry Societies·J AufenangerR Ensenauer
Jan 1, 1993·Archives of Insect Biochemistry and Physiology·J M Uscian, D W Stanley-Samuelson
Feb 22, 2002·Archives of Insect Biochemistry and Physiology·D Sun, J E Steele
Jun 13, 2002·European Journal of Clinical Investigation·Werner JarossMario Menschikowski
Apr 15, 1989·European Journal of Biochemistry·J PfeilschifterI Wiesenberg
Jun 15, 1995·European Journal of Biochemistry·H G SahlG Bierbaum
Feb 1, 1989·Klinische Wochenschrift·G E Hoffmann, W G Guder
Sep 2, 2005·Journal of the American Society of Nephrology : JASN·Claudia PetryJosef Pfeilschifter

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