Endogenous synthesis of milk oleic acid in dairy ewes: In vivo measurement using 13 C-labeled stearic acid

Journal of Dairy Science
P G ToralG Hervás

Abstract

The use of stable isotopes is a reliable and risk-free alternative to radioactive tracers for directly examining in vivo fatty acid (FA) metabolism. However, very limited information is available in ruminants, and none is available in sheep. Therefore, we conducted an experiment in dairy ewes to determine, for the first time in this species, the uptake, Δ9-desaturation, and secretion of 13C-labeled stearic acid (SA) into milk with the aim of measuring in vivo endogenous synthesis of milk oleic acid (OA) and stearoyl-CoA desaturase activity. Six lactating Assaf ewes fed a total mixed ration (forage:concentrate ratio = 30:70) received an intravenous injection of 2 g of 13C-labeled SA. At -24, -15, 0, 4, 8, 12, 16, 20, 24, 36, 48, 60, and 72 h postinjection (p.i.), milk yield was recorded and milk samples were collected to examine fat concentration and FA composition, including compound-specific isotope analysis of SA and OA by gas chromatography-combustion isotope ratio mass spectrometry. Over the p.i. period, the SA proportion ranged from 7.6 to 8.3% of total FA, with a maximum 13C enrichment of 1.9%, whereas OA was more abundant (14.3-15.4% of total FA) and had lower 13C enrichments (up to 0.69%). On average, 15% of the isotopi...Continue Reading

References

Jan 1, 1980·Progress in Lipid Research·R G Vernon
Oct 1, 1995·Journal of Veterinary Pharmacology and Therapeutics·G ZivA Glickman
Feb 18, 1998·Zentralblatt für Veterinärmedizin. Reihe A·M Shem-TovA Saran
Jun 23, 1999·Journal of Chromatography. a·W Meier-Augenstein
Apr 18, 2002·Journal of Agricultural and Food Chemistry·Alfonso SánchezMartín Esqueda
May 29, 2002·The Journal of Nutritional Biochemistry·Benjamin A. CorlDale E. Bauman
Jun 17, 2006·Journal of Vascular and Interventional Radiology : JVIR·Joseph M Ullman
Apr 14, 2007·Journal of Dairy Science·F GlasserY Chilliard
Jan 11, 2008·Advances in Experimental Medicine and Biology·K J ShingfieldD I Givens
May 13, 2008·Journal of Animal Science·O TaugbølO M Harstad
Jun 12, 2012·The British Journal of Nutrition·Inger J KarlengenOdd M Harstad
Sep 25, 2012·Progress in Lipid Research·Leanne Hodson, Barbara A Fielding
Oct 4, 2012·Animal : an International Journal of Animal Bioscience·K J ShingfieldN D Scollan
Jun 11, 2014·Journal of Dairy Science·J R LoftenA F Kertz
Dec 3, 2015·Journal of Dairy Science·P G ToralP Frutos

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