Endogenous testosterone increases L-type Ca2+ channel expression in porcine coronary smooth muscle

American Journal of Physiology. Heart and Circulatory Physiology
D K BowlesC L Heaps

Abstract

Evidence indicates that gender and sex hormonal status influence cardiovascular physiology and pathophysiology. We recently demonstrated increased L-type voltage-gated Ca2+ current (ICa,L) in coronary arterial smooth muscle (CASM) of male compared with female swine. The promoter region of the L-type voltage-gated Ca2+ channel (VGCC) (Cav1.2) gene contains a hormone response element that is activated by testosterone. Thus the purpose of the present study was to determine whether endogenous testosterone regulates CASM ICa,L through regulation of VGCC expression and activity. Sexually mature male and female Yucatan swine (7-8 mo; 35-45 kg) were obtained from the breeder. Males were left intact (IM, n=8), castrated (CM, n=8), or castrated with testosterone replacement (CMT, n=8; 10 mg/day Androgel). Females remained gonad intact (n=8). In right coronary arteries, both Cav1.2 mRNA and protein were greater in IM compared with intact females. Cav1.2 mRNA and protein were reduced in CM compared with IM and restored in CMT. In isolated CASM, both peak and steady-state ICa were reduced in CM compared with IM and restored in CMT. In males, a linear relationship was found between serum testosterone levels and ICa. In vitro, both testostero...Continue Reading

References

Oct 1, 1976·Annals of Internal Medicine·W B KannelT Gordon
Jul 1, 1990·The American Journal of Physiology·M T NelsonN B Standen
May 1, 1994·Circulation·R H KarasM E Mendelsohn
Nov 1, 1995·Journal of Vascular Research·A D Hughes
Mar 1, 1996·Journal of Cardiovascular Pharmacology·J W Karanian, P W Ramwell
Mar 1, 1997·Journal of Cardiovascular Pharmacology·K HigashiuraP V Halushka
Aug 1, 1997·The Journal of General Physiology·B D JohnsonG M Rubanyi
Nov 15, 1997·The American Journal of Physiology·D K BowlesM Sturek
Dec 9, 1998·The American Journal of Physiology·D K BowlesM Sturek
Mar 11, 1999·The American Journal of Cardiology·C M WebbP Collins
Sep 28, 1999·Clinical and Experimental Pharmacology & Physiology·J K Crews, R A Khalil
Apr 6, 2000·American Journal of Physiology. Heart and Circulatory Physiology·L LiuJ D Marsh
Feb 7, 2001·American Journal of Physiology. Cell Physiology·J G Murphy, R A Khalil
Aug 3, 2000·American Journal of Physiology. Heart and Circulatory Physiology·G G GearyS P Duckles
Mar 15, 2001·Proceedings of the National Academy of Sciences of the United States of America·L NathanG Chaudhuri
Sep 15, 2001·American Journal of Physiology. Heart and Circulatory Physiology·V P DeenadayaluA J Garcia
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Mar 21, 2002·Proceedings of the National Academy of Sciences of the United States of America·Tapan K MukherjeeLauren Nathan
Jul 2, 2002·JAMA : the Journal of the American Medical Association·Deborah GradyUNKNOWN HERS Research Group
Jul 19, 2002·JAMA : the Journal of the American Medical Association·Jacques E RossouwUNKNOWN Writing Group for the Women's Health Initiative Investigators
Apr 10, 2003·American Journal of Physiology. Endocrinology and Metabolism·Kish L GoldenJerome Moulden
May 10, 2003·American Journal of Physiology. Heart and Circulatory Physiology·Ritu ChatrathVirginia M Miller
Jun 6, 2003·Endocrine Reviews·Peter Y LiuDavid J Handelsman
Oct 11, 2003·American Journal of Physiology. Heart and Circulatory Physiology·Rayna J GonzalesSue P Duckles
Jan 31, 2004·Journal of Applied Physiology·D K BowlesE M Price
May 4, 2004·Biochemical and Biophysical Research Communications·Jason L ScraggChris Peers

❮ Previous
Next ❯

Citations

Jan 3, 2013·Clinical and Experimental Pharmacology & Physiology·Paulo A SaldanhaIgnacio Verde
Nov 15, 2011·American Journal of Physiology. Heart and Circulatory Physiology·Viju DeenadayaluRichard E White
Feb 29, 2008·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Lynn A BurnesKathleen A Sluka
Apr 9, 2008·Pediatric Research·Beatrix S TraaAnne M Comi
Jul 17, 2013·Basic Research in Cardiology·Zachary C BerwickJohnathan D Tune
Feb 8, 2011·Cell Calcium·Jonathan SatinShawn M Crump
May 8, 2010·Maturitas·Carolyn M Webb, Peter Collins
Sep 22, 2015·Life Sciences·Neide Hyppolito JurkiewiczMaria Christina Werneck Avellar
Aug 2, 2006·International Journal of Andrology·Philip KumanovGeorgi Kirilov
Apr 21, 2006·Vascular Pharmacology·Joanne HallRichard D Jones
Jan 24, 2006·Vascular Pharmacology·Swapnil SonkusareNancy J Rusch
Apr 12, 2005·Vascular Pharmacology·K K MaddaliD K Bowles
Apr 20, 2013·Journal of Molecular and Cellular Cardiology·Ashraf Said Al MadhounIlona S Skerjanc
May 26, 2012·Channels·Joel M BrittainRajesh Khanna
Apr 29, 2015·The Journal of General Physiology·Osama F HarrazDonald G Welsh
Feb 7, 2006·Journal of the American College of Cardiology·Carl J PepineC Noel Bairey Merz
Sep 8, 2018·American Journal of Physiology. Heart and Circulatory Physiology·Mallikarjuna R PabbidiSumit P Sontakke
Jun 27, 2006·American Journal of Physiology. Heart and Circulatory Physiology·D L TharpD K Bowles
May 18, 2007·American Journal of Physiology. Cell Physiology·D K BowlesD H Korzick
May 16, 2009·Journal of Pharmacological Sciences·Akitoshi OhnoYuji Imaizumi
May 23, 2014·Menopause : the Journal of the North American Menopause Society·Darla L TharpDouglas K Bowles
Oct 4, 2017·Pharmacology Research & Perspectives·Brent J F HillNancy J Rusch
Nov 11, 2014·Nihon yakurigaku zasshi. Folia pharmacologica Japonica·Jumpei Ito, Andrés Daniel Maturana
Aug 2, 2018·The Aging Male : the Official Journal of the International Society for the Study of the Aging Male·L Y MorgunovV I Skvortsova
Dec 11, 2019·Journal of Cardiovascular Translational Research·Margarida LorigoElisa Cairrao
Jul 23, 2021·Cardiovascular Research·Antonio CittadiniAndrea Salzano

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme
Kish L GoldenY Jiang
American Journal of Physiology. Regulatory, Integrative and Comparative Physiology
Julia M Orshal, R A Khalil
© 2022 Meta ULC. All rights reserved