Endogenous ubiquinol prevents protein modification accompanying lipid peroxidation in beef heart submitochondrial particles

Free Radical Biology & Medicine
P Forsmark-AndréeL Ernster

Abstract

This article is a study of the relationship between lipid peroxidation and protein modification in beef heart submitochondrial particles, and the protective effect of endogenous ubiquinol (reduced coenzyme Q) against these effects. ADP-Fe3+ and ascorbate were used to initiate lipid peroxidation and protein modification, which were monitored by measuring TBARS and protein carbonylation, respectively. Endogenous ubiquinone was reduced by the addition of succinate and antimycin. The parameters investigated included extraction and reincorporation of ubiquinone, and comparison of the effect of ubiquinol with those of various antioxidant compounds and enzymes, as well as the iron chelator EDTA. Under all conditions employed there was a close correlation between lipid peroxidation and protein carbonylation, and the inhibition of these effects by endogenous ubiquinol. SDS-PAGE analysis revealed a differential effect on individual protein components and its prevention by ubiquinol. Conceivable mechanisms behind the observed oxidative modifications of membrane phospholipids and proteins and of the role of ubiquinol in preventing these effects are considered.

References

Dec 15, 1992·Biochemical and Biophysical Research Communications·A Z ReznickL Packer
May 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·K Uchida, E R Stadtman
Jun 1, 1992·Archives of Biochemistry and Biophysics·F AbergL Ernster
Feb 14, 1992·Archives of Biochemistry and Biophysics·J R Palamanda, J P Kehrer
Jan 1, 1991·Annals of Medicine·H EsterbauerH Rabl
Aug 15, 1991·Archives of Biochemistry and Biophysics·N L ParinandiH H Schmid
Jan 1, 1991·Free Radical Biology & Medicine·H EsterbauerH Zollner
Jan 1, 1990·Methods in Enzymology·R L LevineE R Stadtman
Mar 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·R StockerB Frei
Jan 1, 1991·Molecular Aspects of Medicine·R Dean, J Simpson
Feb 1, 1990·Acta Physiologica Scandinavica·B SoussiA C Bylund-Fellenius
Jan 1, 1990·Free Radical Research Communications·V Narayanaswami, H Sies
Aug 15, 1989·Biochemical and Biophysical Research Communications·J V Hunt, R T Dean
Jan 1, 1988·Free Radical Biology & Medicine·K J Davies, S W Lin
Feb 1, 1985·Archives of Biochemistry and Biophysics·T OkayasuJ L Farber
Mar 21, 1986·Biochimica Et Biophysica Acta·H EsterbauerM Comporti
Feb 15, 1988·The Biochemical Journal·J V HuntR T Dean
Oct 8, 1987·Biochimica Et Biophysica Acta·A KalénG Dallner
Dec 1, 1986·Archives of Biochemistry and Biophysics·A W GirottiJ E Jordan
Feb 15, 1985·Biochemical and Biophysical Research Communications·R T Dean, J K Pollak
Dec 13, 1982·Biochimica Et Biophysica Acta·L MészárosI Horváth
May 28, 1980·Biochimica Et Biophysica Acta·M W SmithB F Trump
Nov 1, 1994·Free Radical Biology & Medicine·J J Chen, B P Yu
Jan 1, 1994·Methods in Enzymology·R L LevineE Shacter
Jan 1, 1994·Methods in Enzymology·K Uchida, E R Stadtman
Jan 1, 1993·Toxicology and Industrial Health·E R StadtmanS G Rhee

❮ Previous
Next ❯

Citations

Dec 30, 2003·BioFactors·Hans NohlLars Gille
Jun 19, 2009·Extremophiles : Life Under Extreme Conditions·Rob Uche OnyenwokeJuergen Wiegel
Jun 12, 2009·Cellular and Molecular Neurobiology·Anwar MasoudRajat Sandhir
Mar 4, 2014·Archives of Biochemistry and Biophysics·Brunhild M HalmAdrian A Franke
Apr 27, 2001·Mechanisms of Ageing and Development·G Spiteller
Jun 25, 2002·Molecular Aspects of Medicine·Malcolm J JacksonSiân B Astley
Mar 5, 2003·Free Radical Biology & Medicine·Magnus BentingerEwa Swiezewska
Jan 1, 1997·Free Radical Biology & Medicine·H P Ciolino, R L Levine
Nov 24, 1999·Free Radical Biology & Medicine·M TomasettiR Alleva
Feb 21, 2008·The British Journal of Nutrition·Michihiro KonIchiro Kono
Dec 16, 2006·Reproduction, Nutrition, Development·Bernard AurousseauDenys Durand
Jul 23, 1996·Proceedings of the National Academy of Sciences of the United States of America·T Q DoC F Clarke
Jun 8, 2000·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·R PamplonaG Barja
Jun 29, 2002·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·R WeindruchS R Spindler
May 25, 2010·Journal of Nutritional Science and Vitaminology·Jung-Mi Kim, Eunju Park
Aug 28, 2009·The Journal of Nutrition·Nathalie SumienMichael J Forster
Feb 24, 2016·Frontiers in Pharmacology·Valeria ContiAmelia Filippelli
Feb 2, 2016·Trends in Cell Biology·Ying Wang, Siegfried Hekimi
Oct 4, 2011·Mutation Research·Constance Schmelzer, Frank Döring
Dec 21, 2010·Experimental Neurology·P A Adlard, A I Bush
May 8, 2007·Mitochondrion·Magnus BentingerGustav Dallner
Jun 4, 1999·Biochimica Et Biophysica Acta·T KishiD J Morré
Mar 14, 2000·Redox Report : Communications in Free Radical Research·A K RainaM A Smith
Feb 1, 2014·Biochimica Et Biophysica Acta·Laurent AusselFrédéric Barras
Apr 27, 2010·Biochimica Et Biophysica Acta·Andrea DlaskováRichard K Porter
Feb 6, 2004·Biochimica Et Biophysica Acta·Mikael TurunenGustav Dallner
Jun 6, 2012·Free Radical Biology & Medicine·Maria E LönnRoland Stocker
Aug 26, 1998·Biochimica Et Biophysica Acta·G Lenaz
Feb 17, 2000·Biochimica Et Biophysica Acta·A BridgeD J Morré
Mar 8, 2016·Photochemistry and Photobiology·Daniel SchniertshauerJörg Bergemann
Nov 12, 2013·Cancer Cell International·Xu-Feng QiSoo-Ki Kim
Aug 4, 2004·Experimental Gerontology·Sergey Kamzalov, Rajindar S Sohal
Aug 6, 2016·Organic & Biomolecular Chemistry·Ikuo NakanishiShunichi Fukuzumi
Jul 9, 2008·Acta Oto-laryngologica·Yoshinobu HiroseHiroshi Yamashita

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.