Endogenously elicited antibodies to platelet derived growth factor-BB and platelet cytosolic protein inhibit aortic lesion development in the cholesterol-fed rabbit

International Journal of Experimental Pathology
C RutherfordG A Ferns

Abstract

Several studies have indicated that growth factors, such as platelet derived growth factor (PDGF), may be important in atherogenesis. These factors are released from platelets, or expressed by cells of the arterial wall. In order to study their role in atherogenesis more directly, rabbits were immunized with PDGF-BB, platelet cytosolic protein, or human serum albumin (HSA), until high titres of antibody were attained. Atherosclerotic lesions were subsequently induced by feeding the animals with a 2% cholesterol enriched diet. At the end of approximately 3 months, the extent of aortic lesion development was assessed by image analysis of en face preparations of aortae stained with Oil Red-O, and histological segments of aortae taken at the level of the first intercostal artery branch point. The endogenous antibodies were characterized with respect to their cross-reactivity, and ability to neutralize PDGF and platelet cytosol-induced cell proliferation and migration in vitro. The endogenous, anti-PDGF-BB antibody was isoform specific, and neutralized the mitogenic and chemotactic properties of PDGF-BB and rabbit platelet cytosolic protein in vitro. The anti-platelet cytosol antibody partially inhibited the chemotactic and mitogeni...Continue Reading

Citations

Oct 2, 2015·Human & Experimental Toxicology·M A NaderM S Zaghloul
Sep 21, 2000·The Proceedings of the Nutrition Society·G A Ferns
Jul 31, 2020·Journal of Mathematical Biology·Michael G WatsonMary R Myerscough
Oct 3, 1999·Physiological Reviews·C H Heldin, B Westermark
Nov 26, 2008·American Journal of Physiology. Heart and Circulatory Physiology·James A ThomasGary K Owens
Nov 13, 2001·International Journal of Experimental Pathology·D J LambG A Ferns
Jul 13, 2017·Journal of Cellular and Molecular Medicine·Tzu-Ming WangSuh-Hang H Juo
Jun 24, 2003·Atherosclerosis·David J LambGordon A A Ferns
Jun 23, 2004·Cytokine & Growth Factor Reviews·Elaine W Raines
Sep 15, 2006·Mayo Clinic Proceedings·Ricardo H AlvarezJorge E Cortes

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