Endomembrane proteomics reveals putative enzymes involved in cell wall metabolism in wheat grain outer layers

Journal of Experimental Botany
Anne-Laure Chateigner-BoutinC Larré

Abstract

Cereal grain outer layers fulfil essential functions for the developing seed such as supplying energy and providing protection. In the food industry, the grain outer layers called 'the bran' is valuable since it is rich in dietary fibre and other beneficial nutriments. The outer layers comprise several tissues with a high content in cell wall material. The cell wall composition of the grain peripheral tissues was investigated with specific probes at a stage of active cell wall synthesis. Considerable wall diversity between cell types was revealed. To identify the cellular machinery involved in cell wall synthesis, a subcellular proteomic approach was used targeting the Golgi apparatus where most cell wall polysaccharides are synthesized. The tissues were dissected into outer pericarp and intermediate layers where 822 and 1304 proteins were identified respectively. Many carbohydrate-active enzymes were revealed: some in the two peripheral grain fractions, others only in one tissue. Several protein families specific to one fraction and with characterized homologs in other species might be related to the specific detection of a polysaccharide in a particular cell layer. This report provides new information on grain cell walls and ...Continue Reading

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Citations

Aug 8, 2017·International Journal of Molecular Sciences·Jundi LiuXin-Qiang He
May 17, 2017·The Plant Journal : for Cell and Molecular Biology·Natalie S BettsGeoffrey B Fincher
Aug 12, 2020·The Plant Journal : for Cell and Molecular Biology·Cornelia M HooperA Harvey Millar
Mar 30, 2017·Frontiers in Plant Science·Lam Dai VuIve De Smet
Jan 8, 2020·International Journal of Molecular Sciences·Mehdi CherkaouiMathilde Francin-Allami
Dec 9, 2020·Plant Science : an International Journal of Experimental Plant Biology·Yves VerhertbruggenAnne-Laure Chateigner-Boutin
Mar 30, 2021·Plant Science : an International Journal of Experimental Plant Biology·Anne-Laure Chateigner-BoutinFabienne Guillon

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Methods Mentioned

BETA
biopsy

Software Mentioned

CCoAOMT
CAZymes Analysis Toolkit
Xcalibur
BLAST
Tandem Spectrum Modeler
COMT
CAD
tBLASTN

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