Endometrial nitric oxide production and nitric oxide synthases in the equine endometrium: Relationship with microvascular density during the estrous cycle

Domestic Animal Endocrinology
Rosário P Roberto da CostaDariusz J Skarzynski

Abstract

Nitric oxide (NO) plays an important role in angiogenesis and in the regulation of the blood flow. This study was carried out to investigate (i) the effects of endogenous estrogens and progestins and exogenous progesterone (P(4)) (5 ng/ml or 1 microg/ml) or estradiol 17beta (E(2)beta) (50 pg/ml or 1 microg/ml) on in vitro endometrial NO synthesis; (ii) the presence of different isoforms of NO synthase; (iii) and their relationship to microvascular density in the equine endometrium during the estrous cycle. NOS expression was also evaluated in the myometrium. Expression of endothelial and inducible forms of NOS in the uterus was assessed by Western blot and immunocytochemistry. Vascular density in endometrial tissue was determined on histologic sections. In the luteal phase, compared to the follicular phase, endometrial NO production increased without exogenous hormones and with exogenous E(2)beta (1 microg/ml). Although immunocytochemistry revealed iNOS and eNOS expression in the endometrium, no positive signal for iNOS was detected by Western blot. Endothelial NOS was observed in endometrial glands, endothelial cells, fibroblasts, blood and lymphatic vessels. Endometrial eNOS expression was the highest in the follicular and mi...Continue Reading

References

May 1, 1990·Endocrine Reviews·C L Clarke, R L Sutherland
Nov 1, 1995·American Journal of Obstetrics and Gynecology·J P Figueroa, G A Massmann
Jan 1, 1995·Human Reproduction·J F TelferI T Cameron
Sep 21, 1995·Nature·S H Snyder
Jul 15, 1993·Biochemical and Biophysical Research Communications·E S NatuzziR K Riemer
Aug 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·D SalveminiP Needleman
Nov 1, 1993·American Journal of Obstetrics and Gynecology·S M SladekJ M Roberts
Feb 1, 1994·Annals of Internal Medicine·C J LowensteinS H Snyder
Jun 11, 1998·Human Reproduction Update·M RosselliR K Dubey
Feb 23, 1999·Human Reproduction Update·I T Cameron, S Campbell
May 14, 1999·Arteriosclerosis, Thrombosis, and Vascular Biology·T MuroharaJ M Isner
May 1, 2001·American Journal of Veterinary Research·G M Ferreira-DiasJ R Silva
Dec 11, 2002·The Journal of Pathology·Ester W J A AlbrechtHarry van Goor
Feb 28, 2003·Biology of Reproduction·Dariusz J SkarzynskiWilliam Hansel
Jan 9, 2004·Fertility and Sterility·M Cecilia JohnsonMargarita Vega
Jul 1, 2004·The Journal of Reproduction and Development·Izabela Woclawek-PotockaDariusz J Skarzynski
Apr 19, 2005·American Journal of Reproductive Immunology : AJRI·Abdorrahman S AlghamdiMats H T Troedsson
Sep 6, 2005·Domestic Animal Endocrinology·G Ferreira-DiasJ A Medeiros

❮ Previous
Next ❯

Citations

Dec 14, 2007·Reproduction, Fertility, and Development·R P Roberto da CostaG Ferreira-Dias
Feb 1, 2014·The Journal of Endocrinology·Anna Z SzóstekDariusz J Skarzynski
Dec 17, 2008·Reproduction : the Official Journal of the Society for the Study of Fertility·M CarvalhoG Ferreira-Dias
Mar 30, 2016·Theriogenology·Maria Fernanda S ReghiniMarco Antonio Alvarenga
Mar 1, 2012·Journal of Reproductive Immunology·Magdalena MajewskaDariusz J Skarzynski
Sep 22, 2018·Reproduction in Domestic Animals = Zuchthygiene·Ana AmaralGraça Ferreira-Dias
Jan 24, 2008·American Journal of Reproductive Immunology : AJRI·Deborah NashI Martin Sheldon
Oct 19, 2012·Reproduction, Fertility, and Development·A GalvãoG Ferreira-Dias
Jul 9, 2020·Anatomia, histologia, embryologia·Juliane RiegerJohanna Plendl
Aug 2, 2008·Reproduction, Fertility, and Development·Rosário P Roberto da CostaGraça Ferreira-Dias
Jan 21, 2021·Animals : an Open Access Journal From MDPI·Ana AmaralGraça Ferreira-Dias
Aug 28, 2017·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Marwa M M Refaie, Maram El-Hussieny
Jun 3, 2021·International Journal of Molecular Sciences·Ana AmaralGraça Ferreira-Dias

❮ Previous
Next ❯

Related Concepts

Related Feeds

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Arterial-Venous in Development & Disease

Arterial-venous development may play a crucial role in cardiovascular diseases. Here is the latest research.