May 12, 2017

Endometrial Stromal and Epithelial Cells Exhibit Unique Aberrant Molecular Defects in Patients With Endometriosis

Reproductive Sciences
Philip C LoganNam D Tran

Abstract

Endometriosis is a chronic inflammatory disease that causes pain and infertility in women of reproductive age. To investigate the pathologic pathways in endometrial stromal and epithelial cells that contribute to the manifestation of endometriosis. In vitro cellular and molecular analyses of isolated eutopic endometrial stromal and epithelial cells. Eutopic stromal and epithelial cells from endometriotic and normal patients were isolated by fluorescence-activated cell sorting for paired sibling RNA sequencing and microRNA microarray. Aberrant pathways were identified using ingenuity pathway analysis networks and confirmed with in vitro modulation of the affected pathways in stromal and epithelial cell cultures. Both stromal versus epithelial cell types and paired endometriotic versus normal samples exhibited distinct hierarchical clustering. Compared to normal samples, there were 151 and 215 differentially expressed genes in the endometriotic stromal and epithelial populations, respectively, and concomitantly 9 and 16 differentially expressed microRNAs. Overall, endometriotic stromal and epithelial cells revealed distinct defects. In endometriotic stromal cells, key decidualization genes Zinc finger E-box Binding protein 1 (ZEB...Continue Reading

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Mentioned in this Paper

Zinc Fingers
Recombinant Transforming Growth Factor
Biochemical Pathway
Microarray Analysis
ZEB1 protein, human
Transforming Growth Factor beta
IL15
Chronic Inflammatory Disorder
TGFA protein, human
Genes

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