Endometriotic lesions synthesize and secrete a haptoglobin-like protein

Biology of Reproduction
K L Sharpe-TimmsR L Zimmer

Abstract

To explore the identity and possible function of endometriosis protein-I (ENDO-I), which is an acidic glycoprotein synthesized and secreted by endometriotic lesions, partial amino acid sequence and cDNA sequence were determined. Partially purified, de novo-synthesized rat endometriosis glycoproteins were separated by two-dimensional SDS-PAGE, transferred to polyvinyl difluoride membranes, and stained with Coomassie blue. Protein corresponding to the size and pI of ENDO-I was cut from the membranes and analyzed by automated Edman degradation. ENDO-I amino acid sequence analysis identified 15 residues that shared significant homology with the beta-chain of rat, mouse, and human haptoglobin (Hp) and human Hp-related protein. Western blot analyses using anti-Hp antibody demonstrated cross-reactivity with de novo-synthesized ENDO-I protein in endometriosis culture media. For nucleotide sequence analysis, poly A-enriched mRNA was isolated from rat endometriotic tissues. A gene-specific oligonucleotide primer was designed and used for 3' rapid amplification of cDNA ends (RACE). Automated sequencing of RACE cDNA fragments identified 859 base pairs, of which 858 were identical to rat Hp. Reverse transcription-polymerase chain reaction w...Continue Reading

Citations

Feb 3, 2012·Cell and Tissue Research·Julie A W StilleyKathy L Sharpe-Timms
May 4, 2005·Fertility and Sterility·Simone FerreroJurgis Gediminas Grudzinskas
Jan 10, 2002·Fertility and Sterility·Kathleen M EysterKeith A Hansen
May 16, 2002·Fertility and Sterility·Anne Van LangendoncktJacques Donnez
Oct 10, 2002·Fertility and Sterility·Kathy L Sharpe-TimmsGary M Horowitz
Apr 20, 2001·European Journal of Obstetrics, Gynecology, and Reproductive Biology·D VinatierP Dufour
Jun 28, 2000·European Journal of Obstetrics, Gynecology, and Reproductive Biology·D VinatierP Dufour
May 14, 2010·Human Reproduction Update·K E MayC M Becker
May 30, 2008·Molecular Human Reproduction·S DefrèreA Van Langendonckt
Apr 13, 2002·Annals of the New York Academy of Sciences·Kathy L Sharpe-Timms
Jan 20, 2012·Journal of Visualized Experiments : JoVE·Katherine E PelchSusan C Nagel
Oct 22, 2008·Expert Review of Proteomics·Simone FerreroJurgis Gedis Grudzinskas
May 29, 2009·Fertility and Sterility·Katherine E PelchSusan C Nagel
Jul 12, 2003·American Journal of Reproductive Immunology : AJRI·Ming-Yih Wu, Hong-Nerng Ho
Jul 13, 2005·Fertility and Sterility·Kathy L Sharpe-Timms
Feb 13, 2014·Journal of Biophotonics·Yichen DingChanghui Li
Jul 24, 2012·Fertility and Sterility·Richard O Burney, Linda C Giudice
Nov 16, 2004·Lancet·Linda C Giudice, Lee C Kao
Oct 6, 2009·Reproductive Sciences·Henda NabliKathy L Sharpe-Timms
Sep 23, 2006·Journal of the Society for Gynecologic Investigation·Murat UlukusAydin Arici
Oct 12, 2001·Annals of the New York Academy of Sciences·K L Sharpe-Timms
Feb 24, 2001·The Veterinary Record·P D EckersallJ L Fitzpatrick

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