Endomucin, a sialomucin expressed in high endothelial venules, supports L-selectin-mediated rolling

International Immunology
Hidenobu KandaM Miyasaka

Abstract

Lymphocyte homing to lymph nodes is regulated by transient but specific interactions between lymphocytes and high endothelial venules (HEVs), the initial phase of which is mainly governed by the leukocyte adhesion molecule L-selectin, which recognizes sulfated and sialylated O-linked oligosaccharides displayed on sialomucin core proteins. One of the sialomucin proteins, endomucin, is predominantly expressed in vascular endothelial cells of a variety of tissues including the HEVs of lymph nodes; however, whether it functions as a ligand for L-selectin remains to be formally proven. Here we show that the endomucin splice isoform a is predominantly expressed in PNAd+ HEVs and MAdCAM-1+ HEVs, as seen in non-HEV-type vascular endothelial cells. Using affinity purification with soluble L-selectin, we found that HEV endomucin is specifically modified with L-selectin-reactive oligosaccharides and can bind L-selectin as well as an HEV-specific mAb, MECA-79. Our results also indicated that a 90-100 kDa endomucin species is preferentially decorated with L-selectin-reactive sugar chains, whereas an 80 kDa species represents conventional forms expressed in non-HEV-type vascular endothelial cells in lymph nodes. Furthermore, a CHO cell line ...Continue Reading

References

Jan 1, 1992·Cold Spring Harbor Symposia on Quantitative Biology·L A LaskyS D Rosen
Mar 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·T K KishimotoE C Butcher
Dec 16, 1993·Nature·E L BergE C Butcher
Oct 1, 1995·The Journal of Cell Biology·K D PuriT A Springer
Oct 15, 1993·Science·S BaumheterL A Lasky
Apr 5, 1996·Science·E C Butcher, L J Picker
Apr 25, 1997·Journal of Immunological Methods·C R BertozziS D Rosen
Jan 29, 1999·Physiological Reviews·D Vestweber, J E Blanks
Nov 26, 1999·Proceedings of the National Academy of Sciences of the United States of America·V RamachandranR P McEver
Dec 11, 1999·International Immunology·D IzawaM Miyasaka
Sep 14, 2001·Biochemical and Biophysical Research Communications·M UenoT Taga
Dec 18, 2001·Developmental Dynamics : an Official Publication of the American Association of Anatomists·G BrachtendorfD Vestweber
Jan 22, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Koichi SaitoMasayuki Miyasaka
May 10, 2002·The American Journal of Pathology·Ulrike SamulowitzDietmar Vestweber
Dec 18, 2002·The Journal of Investigative Dermatology·Annegret KuhnDietmar Vestweber

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Citations

Nov 30, 2005·The Journal of Experimental Medicine·Azusa MatsubaraHiromitsu Nakauchi
Apr 24, 2013·Monoclonal Antibodies in Immunodiagnosis and Immunotherapy·Xiaomei JiangChongjie Zhang
Aug 9, 2012·Tissue Engineering. Part a·Michael J MienaltowskiDavid E Birk
Sep 8, 2016·Proceedings of the National Academy of Sciences of the United States of America·Imtiaz Iftakhar-E-KhudaSirpa Jalkanen
Apr 18, 2017·Proceedings of the Japan Academy. Series B, Physical and Biological Sciences·Akira TakedaMasayuki Miyasaka
Jul 12, 2018·Applied Immunohistochemistry & Molecular Morphology : AIMM·Angel Fernandez-FloresCarlos Monteagudo
Dec 17, 2010·The Journal of Rheumatology·Kazumasa NishimotoShigeki Momohara
Nov 6, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Masanori MatsumotoTakako Hirata
Dec 13, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Masanori MatsumotoTakako Hirata
Nov 6, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Danielle L DraytonNancy H Ruddle
May 20, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Tamás CzömpölyPéter Balogh
Jul 26, 2005·Biochimica Et Biophysica Acta·Judy R van Beijnum, Arjan W Griffioen

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