Endomucin prevents leukocyte-endothelial cell adhesion and has a critical role under resting and inflammatory conditions

Nature Communications
Alisar ZahrPablo Argüeso

Abstract

Endomucin is a membrane-bound glycoprotein expressed luminally by endothelial cells that line postcapillary venules, a primary site of leukocyte recruitment during inflammation. Here we show that endomucin abrogation on quiescent endothelial cells enables neutrophils to adhere firmly, via LFA-1-mediated binding to ICAM-1 constitutively expressed by endothelial cells. Moreover, TNF-α stimulation downregulates cell surface expression of endomucin concurrent with increased expression of adhesion molecules. Adenovirus-mediated expression of endomucin under inflammatory conditions prevents neutrophil adhesion in vitro and reduces the infiltration of CD45(+) and NIMP-R14(+) cells in vivo. These results indicate that endomucin prevents leukocyte contact with adhesion molecules in non-inflamed tissues and that downregulation of endomucin is critical to facilitate adhesion of leukocytes into inflamed tissues.

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Citations

Apr 12, 2018·Annual Review of Biomedical Engineering·Laura Niklason, Guohao Dai
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Methods Mentioned

BETA
PCR
confocal microscopy
flow cytometry
glycosylation
fluorescence microscopy
protein assay
electrophoresis
transfection
biopsy
restriction digest

Software Mentioned

Adobe Photoshop
Prism
ImageJ
FlowJo
Videolab

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