PMID: 18723642Aug 30, 2008Paper

Endonuclease activity of phenol oxidase from Musca domestica larvae

The Biological Bulletin
Shaoguang SunShunzhang Qi

Abstract

Phenol oxidase (PO), a copper-containing enzyme with oxygenase activity, can convert mono- or diphenol into quinone and plays an important role in the arthropod melanization reaction. Here, we report a new property of PO from Musca domestica larvae: a thermotolerant endonuclease activity, by which PO can degrade plasmid DNA even after being heated to 80 degrees C for 20 min. We cloned PO cDNA, constructed the expression vector pVAX1-PO, and expressed it in HeLa cells. The expression product showed the same properties as purified PO. Our data indicate that PO is a bifunctional enzyme, exhibiting both oxygenase and endonuclease activity, suggesting new roles for this important molecule in the innate responses of M. domestica.

Citations

Jul 15, 2015·G3 : Genes - Genomes - Genetics·Dianxiang LiYuanyuan Luan

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