PMID: 9422591Jan 9, 1998Paper

Endonuclease V (nfi) mutant of Escherichia coli K-12

Journal of Bacteriology
G Guo, B Weiss

Abstract

Endonuclease V (deoxyinosine 3' endonuclease), the product of the nfi gene, has a specificity that encompasses DNAs containing dIMP, abasic sites, base mismatches, uracil, and even untreated single-stranded DNA. To determine its importance in DNA repair pathways, nfi insertion mutants and overproducers (strains bearing nfi plasmids) were constructed. The mutants displayed a twofold increase in spontaneous mutations for several markers and an increased sensitivity to killing by bleomycin and nitrofurantoin. An nfi mutation increased both cellular resistance to and mutability by nitrous acid. This agent should generate potential cleavage sites for the enzyme by deaminating dAMP and dCMP in DNA to dIMP and dUMP, respectively. Relative to that of a wild-type strain, an nfi mutant displayed a 12- to 1,000-fold increase in the frequency of nitrite-induced mutations to streptomycin resistance, which are known to occur in A x T base pairs. An nfi mutation also enhanced the lethality caused by a combined deficiency of exonuclease III and dUTPase, which has been attributed to unrepaired abasic sites. However, neither the deficiency nor the overproduction of endonuclease V affected the growth of the single-stranded DNA phages M13 or phiX1...Continue Reading

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Citations

Apr 13, 2006·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Daniel J TurnerFrancis Barany
Aug 8, 2009·FEMS Microbiology Reviews·Bjørn DalhusMagnar Bjørås
Oct 16, 2015·Proceedings of the National Academy of Sciences of the United States of America·Patricia L FosterHaixu Tang
Jun 29, 2006·Molecular and Cellular Biochemistry·A P M NunesA Caldeira-de-Araujo
Nov 7, 2008·Journal of Radiation Research·Shin-Ichiro YonekuraQiu-Mei Zhang-Akiyama
Jan 13, 2009·Nature Structural & Molecular Biology·Bjørn DalhusMagnar Bjørås
Dec 25, 2012·Cellular and Molecular Life Sciences : CMLS·Weiguo Cao
Jun 30, 2009·Journal of Bacteriology·Young-Man Kwon, Bernard Weiss
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Jun 7, 2003·Molecular Microbiology·Jill S Bradshaw, Andrei Kuzminov
Feb 10, 2010·Proceedings of the National Academy of Sciences of the United States of America·Cheryl L ClausonPaul W Doetsch
Sep 6, 2006·Journal of Bacteriology·Brian Budke, Andrei Kuzminov
Feb 19, 2014·Journal of Biochemistry·Shinichi KiyonariYoshizumi Ishino
Aug 19, 2017·Scientific Reports·Daniel García-CaballeroDolores González-Pacanowska
Nov 9, 2006·The Journal of Biological Chemistry·Nicholas E Burgis, Richard P Cunningham
Oct 4, 2002·Free Radical Biology & Medicine·Yoke W Kow
Feb 1, 2013·EcoSal Plus·Sophie CouvéMurat Saparbaev
Sep 8, 1998·Microbiology and Molecular Biology Reviews : MMBR·M K Berlyn

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