Endophilin-A coordinates priming and fusion of neurosecretory vesicles via intersectin.

Nature Communications
Sindhuja GowrisankaranIra Milosevic

Abstract

Endophilins-A are conserved endocytic adaptors with membrane curvature-sensing and -inducing properties. We show here that, independently of their role in endocytosis, endophilin-A1 and endophilin-A2 regulate exocytosis of neurosecretory vesicles. The number and distribution of neurosecretory vesicles were not changed in chromaffin cells lacking endophilin-A, yet fast capacitance and amperometry measurements revealed reduced exocytosis, smaller vesicle pools and altered fusion kinetics. The levels and distributions of the main exocytic and endocytic factors were unchanged, and slow compensatory endocytosis was not robustly affected. Endophilin-A's role in exocytosis is mediated through its SH3-domain, specifically via a direct interaction with intersectin-1, a coordinator of exocytic and endocytic traffic. Endophilin-A not able to bind intersectin-1, and intersectin-1 not able to bind endophilin-A, resulted in similar exocytic defects in chromaffin cells. Altogether, we report that two endocytic proteins, endophilin-A and intersectin-1, are enriched on neurosecretory vesicles and regulate exocytosis by coordinating neurosecretory vesicle priming and fusion.

References

Jun 1, 1996·The Journal of Cell Biology·K TakeiP De Camilli
Mar 28, 1997·The Journal of Biological Chemistry·E de HeuvelP S McPherson
Aug 5, 1997·Proceedings of the National Academy of Sciences of the United States of America·N RingstadP De Camilli
Dec 31, 1997·The Journal of Cell Biology·C Smith, E Neher
Nov 13, 1998·The Journal of Biological Chemistry·M YamabhaiB K Kay
May 21, 1999·The Journal of Biological Chemistry·N K HussainP S McPherson
Aug 24, 2001·The Journal of Biological Chemistry·N RingstadP De Camilli
Oct 18, 2001·The Journal of Cell Biology·K FarsadP De Camilli
Nov 20, 2002·Annals of the New York Academy of Sciences·Marie-France BaderNicolas Vitale
Dec 18, 2002·Proceedings of the National Academy of Sciences of the United States of America·Phillip HolroydReinhard Jahn
Mar 26, 2003·Nature Cell Biology·Gregor DernickManfred Lindau
Nov 19, 2003·Neuron·Kimberly R SchuskeErik M Jorgensen
Jun 9, 2004·Methods : a Companion to Methods in Enzymology·Dieter Bruns
Jan 26, 2005·The Journal of Cell Biology·Matthew KirkhamRobert G Parton
Mar 11, 2005·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Ira MilosevicErwin Neher
May 28, 2005·The EMBO Journal·Maria BorisovskaDieter Bruns
Aug 25, 2005·Nature Methods·Eugene V Mosharov, David Sulzer
Mar 17, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Abdeladim ElhamdaniCristina R Artalejo
Oct 4, 2006·Pflügers Archiv : European journal of physiology·Erwin Neher
Dec 13, 2006·Proceedings of the National Academy of Sciences of the United States of America·Rushika M PereraDerek Toomre
Jan 6, 2007·PloS One·Heidi de WitMatthijs Verhage
Aug 10, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Attila Gulyás-KovácsJakob B Sørensen
Mar 1, 2008·Proceedings of the National Academy of Sciences of the United States of America·Jean-Sébastien SchonnJakob B Sørensen
May 23, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Yuanyuan LiuJens Rettig
Aug 5, 2008·Human Molecular Genetics·Yong YuMelanie A Pritchard
Jun 2, 2009·Current Biology : CB·Akiko Nakano-KobayashiLinda Van Aelst
Feb 18, 2010·Proceedings of the National Academy of Sciences of the United States of America·Arndt PechsteinVolker Haucke
Aug 13, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Arlek M González-JamettAna M Cárdenas
Nov 10, 2010·Cellular and Molecular Neurobiology·Shyue-An ChanCorey Smith
Dec 25, 2010·Cell Biochemistry and Biophysics·Ole KjaerulffAnita Jung
Mar 12, 2011·Journal of Neurochemistry·Fanny MomboisseStéphane Gasman
Apr 5, 2011·Molecular Biology of the Cell·Arun AnantharamRonald W Holz

❮ Previous
Next ❯

Citations

Jun 27, 2020·The Biochemical Journal·Alessandra Casamento, Emmanuel Boucrot
Feb 6, 2021·Journal of Neurochemistry·Callista B Harper, Karen J Smillie
Apr 15, 2021·Computational and Structural Biotechnology Journal·Xiaowei LiShemin Lu
May 23, 2021·Science Advances·Alessandro MoroMatthijs Verhage
Jun 22, 2021·Journal of Parkinson's Disease·Meir Schechter, Ronit Sharon

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
electron microscopy
pull-down
gel filtration
electrophoresis

Software Mentioned

Imaris
Pulse
Image Studio Lite
EMSIS
Igor Pro Metrics
IGOR Pro
SPSS
HEKA
ImageJ
iTEM

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.