Endophytic fungus Paecilomyces formosus LHL10 produces sester-terpenoid YW3548 and cyclic peptide that inhibit urease and α-glucosidase enzyme activities

Archives of Microbiology
Saqib BilalIn-Jung Lee

Abstract

Endophytic fungi have been used to obtain novel bioactive secondary metabolites with potential applications in medical and agricultural sectors, which can also act as lead targets for pharmaceutical and medicinal potential. In the present study, the endophytic fungus Paecilomyces formosus LHL10 isolated from the root of cucumber plant was tested for its enzyme inhibitory potential. The ethyl acetate (EtOAc) extract of LHL10 was screened for its inhibitory effect on acetylcholinesterase (AChE), α-glucosidase, urease, and anti-lipid peroxidation. The findings suggest that the EtOAc extract from LHL10 possesses significant inhibitory potential against urease and α-glucosidase. The EtOAc extract was thus, subjected to advanced column chromatographic techniques for the isolation of pure compounds. The structure elucidation was carried out through spectroscopic analysis and comparison with literature data, and these compounds were confirmed as known a sester-terpenoid (1) and a known cyclic peptide (2). The enzyme inhibition bioassay indicated that Compounds 1 and 2 exhibited remarkable inhibitory rate against α-glucosidase and urease, with an IC50 value of 61.80 ± 5.7, 75.68 ± 6.2 and 74.25 ± 4.3, 190.5 ± 10.31 µg/g, respectively. T...Continue Reading

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Citations

Jun 5, 2020·Critical Reviews in Food Science and Nutrition·Roberto Castro-MuñozEmilia Gontarek
Sep 23, 2019·Bioprocess and Biosystems Engineering·Barbara Vargas da RosaRaquel Cristine Kuhn
Oct 9, 2020·BMC Complementary Medicine and Therapies·Celiwe Innocentia NxumaloTsolanku Sidney Maliehe
Feb 2, 2021·Journal of Invertebrate Pathology·José Brites-NetoSimone Possedente de Lira
Nov 4, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ze-Bao DaiGuo-Hong Li
May 18, 2021·Biotechnology and Applied Biochemistry·Shivankar AgrawalSunil Kumar Deshmukh
Jun 3, 2021·International Journal of Molecular Sciences·Alicja K SurowiakDaniel J Strub

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Datasets Mentioned

BETA
HQ444388

Methods Mentioned

BETA
nuclear magnetic resonance
column chromatography
NMR

Software Mentioned

BLASTn
SAS
GraphPad

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