Endophytic Paraconiothyrium sp. from Zingiber officinale Rosc. Displays Broad-Spectrum Antimicrobial Activity by Production of Danthron

Current Microbiology
C AnishaE K Radhakrishnan

Abstract

The bioactivity spectrum of fungal endophytes isolated from Zingiber officinale was analyzed against clinical pathogens and against the phytopathogen Pythium myriotylum, which causes Pythium rot in ginger. One of the isolates GFM13 showed broad bioactivity against various pathogens tested including P. myriotylum. The spore suspension as well as the culture filtrate of the endophytic fungal isolate was found to effectively protect ginger rhizomes from Pythium rot. By molecular identification, the fungal endophyte was identified as Paraconiothyrium sp. The bioactive compound produced by the isolate was separated by bioactivity-guided fractionation and was identified by GC-MS as danthron, an anthraquinone derivative. PCR amplification showed the presence of non-reducing polyketide synthase gene (NR-PKS) in the endophyte GFM13, which is reported to be responsible for the synthesis of anthraquinones in fungi. This is the first report of danthron being produced as the biologically active component of Paraconiothyrium sp. Danthron is reported to have wide pharmaceutical and agronomic applications which include its use as a fungicide in agriculture. The broad-spectrum antimicrobial activity of danthron and the endophytic origin of Para...Continue Reading

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Citations

Nov 18, 2020·Toxins·Marco Masi, Antonio Evidente
Jul 3, 2021·Critical Reviews in Microbiology·Junfei WangShaohua Wu

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Datasets Mentioned

BETA
KX247121
KX247126

Methods Mentioned

BETA
Polymerase Chain Reaction
PCR
electrophoresis
column chromatography

Software Mentioned

MEGA
BLAST Basic Local Alignment Search Tool
BLAST

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