Endoplasmic reticulum aminopeptidase 1 (ERAP1) exhibits functionally significant interaction with HLA-B27 and relates to subtype specificity in ankylosing spondylitis

Annals of the Rheumatic Diseases
Nigil HaroonRobert D Inman

Abstract

The functional interaction of endoplasmic reticulum aminopeptidase 1 (ERAP1) with human leucocyte antigen (HLA)-B*27 could be important in the pathogenesis of ankylosing spondylitis (AS). AS is associated with B*27:04 and B*27:05, but not with B*27:06 and B*27:09. The authors studied the surface expression of peptide-HLA(pHLA)-B27 complexes and HLA class-I free heavy chains (FHCs) on peripheral blood mononuclear cells of patients with AS with different ERAP1 single nucleotide polymorphisms. The effects of ERAP1 suppression on HLA-B*27 subtypes were tested. Peripheral blood mononuclear cells were collected from Caucasian patients with AS for flow cytometry and were stained for pHLA and FHCs. Genotyping was performed for two ERAP1 single nucleotide polymorphisms (rs27044(C/G) and rs30187(C/T)). C1R cells transfected with different HLA-B27 subtypes (B*27:04, B*27:05, B*27:06 and B*27:09) were subjected to ERAP1 suppression by small interfering RNA and stained using the monoclonal antibody (mAb) MARB4 as well as antibodies for pHLA, FHC, intracellular FHC (IC-FHC). MARB4 has been reported to bind to HLA-B27 with extended peptides. The authors found variations in FHC expression on the monocytes of patients with AS, depending on diff...Continue Reading

Citations

Aug 7, 2013·Molecular Immunology·Carlos Alvarez-Navarro, José A López de Castro
Aug 25, 2012·Molecular & Cellular Proteomics : MCP·Noel García-MedelJosé A López de Castro
May 16, 2013·Current Opinion in Rheumatology·Barbara Uchanska-ZieglerPeter Schmieder
May 10, 2013·Current Opinion in Rheumatology·Carlos Alvarez-Navarro, José A López de Castro
Dec 10, 2014·Best Practice & Research. Clinical Rheumatology·Rik J Lories, Nigil Haroon
Mar 5, 2015·Modern Rheumatology·Yu XiaYue-Ran Zhao
Oct 7, 2015·Nature Reviews. Rheumatology·Matthew A BrownB Paul Wordsworth
Apr 3, 2013·Molecular Immunology·Efstratios Stratikos, Lawrence J Stern
Mar 5, 2013·Current Opinion in Immunology·Sarah KeidelPaul Wordsworth
Oct 16, 2015·International Journal of Rheumatic Diseases·Abhijeet Danve, James O'Dell
Dec 2, 2014·Immunologic Research·Emma ReevesChristopher J Edwards
Apr 12, 2015·Annual Review of Immunology·Paul Bowness
Apr 28, 2017·Nature Reviews. Rheumatology·Vidya RanganathanNigil Haroon
Apr 8, 2017·Current Rheumatology Reports·Nurullah AkkoçMuhammad A Khan
May 17, 2018·Zeitschrift für Rheumatologie·U Syrbe, X Baraliakos
Jul 29, 2018·Bioscience Reports·Emma Reeves, Edward James
May 17, 2019·Nature Reviews. Rheumatology·Robert BuschElizabeth D Mellins
Nov 13, 2019·Current Rheumatology Reports·Susanne Juhl Pedersen, Walter P Maksymowych
Jun 2, 2018·Rheumatology International·José Pablo Romero-LópezEthel García-Latorre
May 24, 2015·Current Opinion in Rheumatology·Tri M Tran, Robert A Colbert

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