Endoplasmic reticulum dynamics, inheritance, and cytoskeletal interactions in budding yeast

Molecular Biology of the Cell
K L FehrenbacherLiza A Pon

Abstract

The endoplasmic reticulum (ER) in Saccharomyces cerevisiae consists of a reticulum underlying the plasma membrane (cortical ER) and ER associated with the nuclear envelope (nuclear ER). We used a Sec63p-green fluorescent protein fusion protein to study motility events associated with inheritance of cortical ER and nuclear ER in living yeast cells. During M phase before nuclear migration, we observed thick, apparently rigid tubular extensions emanating from the nuclear ER that elongate, undergo sweeping motions along the cell cortex, and shorten. Two findings support a role for microtubules in this process. First, extension of tubular structures from the nuclear ER is inhibited by destabilization of microtubules. Second, astral microtubules, structures that undergo similar patterns of extension, cortical surveillance and retraction, colocalize with nuclear ER extensions. During S and G(2) phases of the cell cycle, we observed anchorage of the cortical ER at the site of bud emergence and apical bud growth. Thin tubules of the ER that extend from the anchored cortical ER display undulating, apparently random movement and move into the bud as it grows. Finally, we found that cortical ER morphology is sensitive to a filamentous acti...Continue Reading

References

Oct 1, 1992·The Journal of Cell Biology·D S Sullivan, T C Huffaker
Jan 1, 1991·Methods in Enzymology·F Sherman
Oct 1, 1986·The Journal of Cell Biology·M TerasakiK Fujiwara
Mar 1, 1984·The Journal of Cell Biology·J V Kilmartin, A E Adams
Mar 1, 1995·The Journal of Cell Biology·B GovindanP Novick
Nov 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·Y Y LiK Bloom
Dec 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·D EshelI R Gibbons
Jul 11, 1998·Molecular Biology of the Cell·S L ShawK Bloom
Aug 12, 1998·The Journal of Cell Biology·F P Finger, P Novick
Sep 29, 1998·The Plant Journal : for Cell and Molecular Biology·P BoevinkC Hawes
Dec 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·N L Catlett, L S Weisman
Jun 3, 1999·The Journal of Cell Biology·J S TirnauerD Pellman
Jun 9, 1999·Current Biology : CB·D L BeachK Bloom
Mar 15, 2001·Proceedings of the National Academy of Sciences of the United States of America·I R BoldoghL A Pon

❮ Previous
Next ❯

Citations

Oct 18, 2002·Current Opinion in Cell Biology·Francis A Barr
Nov 30, 2002·Current Opinion in Microbiology·Daniel SchottAnthony Bretscher
Dec 8, 2011·Nature Reviews. Molecular Cell Biology·John A Hammer, James R Sellers
Jan 5, 2011·The Journal of Biological Chemistry·Tomokazu KuritaKoji Yoda
Apr 30, 2008·The Journal of Biological Chemistry·Yoko ShibataGia K Voeltz
Oct 30, 2013·The Journal of Cell Biology·Paolo ColombiC Patrick Lusk
Apr 21, 2006·Molecular Biology of the Cell·Johan-Owen De CraenePeter Novick
Mar 1, 2013·Molecular Biology of the Cell·Marisa Kirchenbauer, Dimitris Liakopoulos
Oct 12, 2004·Annual Review of Cell and Developmental Biology·David PruyneAnthony Bretscher
May 15, 2013·Cell Structure and Function·Yuki Ishiwata-KimataYukio Kimata
Feb 5, 2008·Genetics·Rebecca L FrederickJanet M Shaw
Dec 15, 2004·Proceedings of the National Academy of Sciences of the United States of America·Catherine A ReinkeBenjamin S Glick
Nov 7, 2007·The Journal of Cell Biology·Christopher J R LoewenTimothy P Levine
Dec 24, 2003·The Journal of Cell Biology·Paula EstradaSusan Ferro-Novick
Dec 24, 2003·The Journal of Cell Biology·Wolfgang Wagner, John A Hammer
Apr 20, 2011·The Journal of Cell Biology·Matt WestGia K Voeltz
Mar 13, 2013·Current Opinion in Cell Biology·Shuliang ChenSusan Ferro-Novick
Apr 29, 2015·Experimental Cell Research·Merja Joensuu, Eija Jokitalo
Jan 8, 2008·Current Opinion in Microbiology·Andrei Fagarasanu, Richard A Rachubinski
Nov 18, 2003·Annual Review of Genetics·Lois S Weisman
Jan 13, 2015·Annual Review of Biochemistry·L M WestrateG K Voeltz
Mar 19, 2014·Nucleus·C Patrick Lusk, Paolo Colombi
Sep 13, 2008·Current Biology : CB·Liza A Pon
Jan 29, 2013·Biochimica Et Biophysica Acta·Orit Hermesh, Ralf-Peter Jansen
Jul 13, 2005·Cell·Dominic HoepfnerHenk F Tabak
Dec 31, 2014·The Journal of Cell Biology·Joachim NellerNils Johnsson
Apr 28, 2016·Developmental Cell·Francisco Javier PiñaMaho Niwa
Jan 11, 2018·The Journal of Cell Biology·Francisco PiñaMaho Niwa
Jul 12, 2002·Yeast

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.