Endoplasmic reticulum, Golgi, and lysosomes are disorganized in lung fibroblasts from chronic obstructive pulmonary disease patients

Physiological Reports
Julie WeidnerEllen Tufvesson

Abstract

Chronic Obstructive Pulmonary Disease (COPD) is often caused by smoking and other stressors. This causes oxidative stress, which induces numerous changes on both the transcriptome and proteome of the cell. We aimed to examine if the endomembrane pathway, including the endoplasmic reticulum (ER), Golgi, and lysosomes, was disrupted in fibroblasts from COPD patients as opposed to healthy ever-smokers or never-smokers, and if the response to stress differed. Different cellular compartments involved in the endomembrane pathway, as well as mRNA expression and apoptosis, were examined before and after the addition of stress in lung fibroblasts from never-smokers, ever-smokers, and patients with COPD. We found that the ER, Golgi, and lysosomes were disorganized in fibroblasts from COPD patients under baseline conditions. After a time course with ER stress inducing chemicals, changes to the phenotypes of cellular compartments in COPD patient fibroblasts were observed, and the expression of the ER stress-induced gene ERP72 was upregulated more in the COPD patient's cells compared to ever-smokers or never-smokers. Lastly, a tendency of increased active Caspase-3 was observed in COPD fibroblasts. Our results show that COPD patients have p...Continue Reading

References

Apr 23, 2003·American Journal of Respiratory Cell and Molecular Biology·Rubin M TuderPeter M Henson
Sep 23, 2006·American Journal of Respiratory Cell and Molecular Biology·Regina Golan-GerstlRaphael Breuer
Feb 4, 2009·The Journal of Pharmacology and Experimental Therapeutics·Wei-Ching HuangChiou-Feng Lin
Nov 27, 2009·COPD·Ali R BehzadDavid C Walker
Feb 20, 2010·International Journal of Cell Biology·Afshin SamaliSanjeev Gupta
Mar 27, 2010·International Journal of Cell Biology·Afshin SamaliSrinivasa M Srinivasula
Jun 4, 2010·The Clinical Respiratory Journal·Gunilla Westergren-ThorssonLeif Bjermer
Jan 27, 2011·Methods in Enzymology·Karen CawleySanjeev Gupta
Jan 27, 2011·Methods in Enzymology·Christine M Oslowski, Fumihiko Urano
May 21, 2011·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Ellen TufvessonLeif Bjermer
Jun 24, 2011·International Journal of Chronic Obstructive Pulmonary Disease·Patrick GeraghtyJeanine M D'Armiento
Jun 16, 2012·Current Molecular Medicine·C M P Ribeiro, W K O'Neal
Aug 1, 2012·Experimental Physiology·Anita Somborac-BacuraAntoon J M van Oosterhout
Aug 2, 2012·The Journal of Clinical Investigation·Rubin M Tuder, Irina Petrache
Nov 22, 2012·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Harshavardhan KencheAnna Blumental-Perry
Jul 16, 2013·Biochimica Et Biophysica Acta·Renata Sano, John C Reed
Apr 8, 2014·Antioxidants & Redox Signaling·Stewart Siyan Cao, Randal J Kaufman
Aug 29, 2014·Toxicological Sciences : an Official Journal of the Society of Toxicology·Poornima MahavadiAndreas Guenther
Oct 5, 2015·Cellular and Molecular Life Sciences : CMLS·Dianne S Schwarz, Michael D Blower
May 4, 2017·Respiratory Research·Julie WeidnerEllen Tufvesson
May 13, 2017·International Journal of Chronic Obstructive Pulmonary Disease·Xiaoyu LiuBaojian Xue

❮ Previous
Next ❯

Citations

Feb 9, 2019·Expert Review of Respiratory Medicine·Young Soon YoonDon D Sin
Apr 2, 2020·Physiological Research·M PokusaA Kralova Trancikova
Jul 10, 2020·European Respiratory Review : an Official Journal of the European Respiratory Society·Martina KorfeiSilke Meiners
Dec 4, 2019·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Zeyu WangQuan Cheng

❮ Previous
Next ❯

Methods Mentioned

BETA
biopsies
PCR
ELISA
glycosylation
electron microscopy
transmission electron microscopy
scanning electron microscopy
electron
fluorescence microscopy

Software Mentioned

Prism
ImageJ

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis