Endoplasmic reticulum protein ERp46 in prostate adenocarcinoma

Oncology Letters
Wilhelmina C M DuivenvoordenJehonathan H Pinthus

Abstract

Endoplasmic reticulum (ER) protein ERp46 is a member of the protein disulfide isomerase family of oxidoreductases, which facilitates the reduction of disulfides in proteins and their folding. Accumulation of misfolded proteins has been implicated in cancer. The objectives of the present study were to investigate the role of ERp46 in prostate cancer, its expression and its effects on prostate cancer growth. A tissue microarray with human prostate cancer and normal prostate tissue samples was stained for ERp46 followed by image analysis. Human prostate adenocarcinoma 22Rv1 cells were stably transfected with short hairpin RNA (shRNA) specific for ERp46, a non-effective scrambled control or a plasmid containing full-length human ERp46 cDNA, and cell growth was determined. Subcloned cells were treated with thapsigargin or tunicamycin to induce ER stress and lysates were subjected to western blot analysis for ER stress proteins. Subcutaneous xenografts of parental 22Rv1, ERp46-overexpressing (ERp46+), shERp46 or scrambled control cells were established in male inbred BALB/c nude mice (n=10/group). Tumor growth curves of the xenografts were constructed over a period of 30 days and subsequently the mice were sacrificed and the amount o...Continue Reading

References

Jul 27, 2002·Annual Review of Cell and Developmental Biology·Heather P HardingDavid Ron
Nov 27, 2008·Proceedings of the National Academy of Sciences of the United States of America·Yong FuAmy S Lee
Jan 21, 2009·Endocrine-related Cancer·Claudio FestucciaMauro Bologna
May 30, 2009·Antioxidants & Redox Signaling·Feras Hatahet, Lloyd W Ruddock
Jan 16, 2010·Biochemical and Biophysical Research Communications·Hayley K CharltonJonathan P Whitehead
Dec 7, 2011·Antioxidants & Redox Signaling·Adam M Benham
Aug 7, 2012·Nature Methods·Anders R KristensenLeonard J Foster
Sep 4, 2012·Cell·Pierre C HavugimanaAndrew Emili
Mar 7, 2014·PloS One·Wilhelmina C M DuivenvoordenJehonathan H Pinthus

❮ Previous
Next ❯

Citations

Mar 24, 2020·Pharmacology & Therapeutics·Andrea G ShergalisNouri Neamati

❮ Previous
Next ❯

Methods Mentioned

BETA
xenograft
transfection
xenografts
protein folding

Software Mentioned

ImageScope
CalcuSyn

Related Concepts

Related Feeds

Cancer Imaging

Imaging techniques, including CT and MR, have become essential to tumor detection, diagnosis, and monitoring. Here is the latest research on cancer imaging.