Endoplasmic reticulum protein targeting of phospholamban: a common role for an N-terminal di-arginine motif in ER retention?

PloS One
Parveen SharmaAnthony O Gramolini

Abstract

Phospholamban (PLN) is an effective inhibitor of the sarco(endo)plasmic reticulum Ca(2+)-ATPase, which transports Ca(2+) into the SR lumen, leading to muscle relaxation. A mutation of PLN in which one of the di-arginine residues at positions 13 and 14 was deleted led to a severe, early onset dilated cardiomyopathy. Here we were interested in determining the cellular mechanisms involved in this disease-causing mutation. Mutations deleting codons for either or both Arg13 or Arg14 resulted in the mislocalization of PLN from the ER. Our data show that PLN is recycled via the retrograde Golgi to ER membrane traffic pathway involving COP-I vesicles, since co-immunoprecipitation assays determined that COP I interactions are dependent on an intact di-arginine motif as PLN RDelta14 did not co-precipitate with COP I containing vesicles. Bioinformatic analysis determined that the di-arginine motif is present in the first 25 residues in a large number of all ER/SR Gene Ontology (GO) annotated proteins. Mutations in the di-arginine motif of the Sigma 1-type opioid receptor, the beta-subunit of the signal recognition particle receptor, and Sterol-O-acyltransferase, three proteins identified in our bioinformatic screen also caused mislocaliza...Continue Reading

References

Dec 1, 1990·Trends in Biochemical Sciences·H R Pelham
Feb 1, 1983·European Journal of Clinical Investigation·F C MartinT J Peters
Jan 1, 1982·Annual Review of Physiology·M Tada, A M Katz
Mar 5, 1996·Proceedings of the National Academy of Sciences of the United States of America·C HarterF Wieland
Jan 1, 1996·Annual Review of Cell and Developmental Biology·R D Teasdale, M R Jackson
Jun 13, 1997·The Journal of Biological Chemistry·Y KimuraD H MacLennan
Sep 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·A SpangL Orci
Oct 28, 1998·Physiological Reviews·H K Simmerman, L R Jones
Jun 22, 1999·The Journal of Biological Chemistry·R A WilkeM B Jackson
Aug 12, 2000·Neuron·M Margeta-MitrovicL Y Jan
Feb 28, 2001·Proceedings of the National Academy of Sciences of the United States of America·N ZerangueB Schwappach
May 10, 2001·The Journal of Clinical Endocrinology and Metabolism·S BordJ Compston
May 4, 2002·Annual Review of Biophysics and Biomolecular Structure·Chris P Ponting, Robert R Russell
Jun 7, 2002·Current Opinion in Neurobiology·Dzwokai Ma, Lily Yeh Jan
Jun 8, 2002·The Journal of Biological Chemistry·Michael R LeachDavid B Williams
Oct 16, 2002·The Journal of Cell Biology·Jia-Shu YangVictor W Hsu
Apr 15, 2003·Proceedings of the National Academy of Sciences of the United States of America·Michio AsahiDavid H MacLennan
May 6, 2003·The Journal of Pharmacology and Experimental Therapeutics·Teruo Hayashi, Tsung-Ping Su
May 24, 2003·Annals of the New York Academy of Sciences·David H MacLennanA Russell Tupling
Jul 3, 2003·Nature Reviews. Molecular Cell Biology·David H MacLennan, Evangelia G Kranias
Nov 24, 2004·Proceedings of the National Academy of Sciences of the United States of America·Anthony O GramoliniDavid H MacLennan
Dec 8, 2004·Nature Cell Biology·Peter WatsonDavid J Stephens
Mar 30, 2005·The Journal of Cell Biology·Wei LiuJennifer Lippincott-Schwartz
Apr 20, 2005·Current Opinion in Structural Biology·Pascal F EgeaPeter Walter
Jan 25, 2006·Proceedings of the National Academy of Sciences of the United States of America·Kobra HaghighiEvangelia G Kranias
Dec 23, 2006·Genome Biology·Zohar ItzhakiHanah Margalit
Feb 9, 2007·American Journal of Physiology. Cell Physiology·David L StenoienDiana J Bigelow
Aug 7, 2007·Annual Review of Genetics·Kara Dolinski, David Botstein
Oct 12, 2007·The Journal of Pharmacology and Experimental Therapeutics·Kyoung-Han KimPeter H Backx
Oct 24, 2007·The Journal of Cell Biology·Kai MichelsenBlanche Schwappach

❮ Previous
Next ❯

Citations

Apr 29, 2011·Cold Spring Harbor Perspectives in Biology·David K Banfield
Aug 13, 2011·The Journal of Biological Chemistry·Philip BidwellSeth L Robia
Jan 31, 2013·The Journal of Biological Chemistry·Przemek A GorskiHoward S Young
Mar 23, 2011·Biotechnology Letters·Yifeng Li
Mar 8, 2011·Expert Opinion on Therapeutic Targets·Teruo HayashiTsung-Ping Su
Jun 4, 2015·Proceedings of the National Academy of Sciences of the United States of America·Allen C T TengAnthony O Gramolini
Jun 19, 2012·Progress in Lipid Research·Qin LiuRandall J Weselake
Feb 22, 2011·Biochemical and Biophysical Research Communications·Anthea J Tennant-EylesRoland G Roberts
Dec 10, 2015·Behavioural Pharmacology·Jonathan L KatzTsung-Ping Su
Dec 14, 2011·Journal of Molecular and Cellular Cardiology·Kobra HaghighiEvangelia G Kranias
Sep 10, 2011·Pharmaceuticals·Jonathan L KatzShang-Yi Tsai
Jun 15, 2018·The Journal of General Physiology·Colline SanchezVincent Jacquemond
Oct 14, 2011·Physical Chemistry Chemical Physics : PCCP·Moutusi Manna, Chaitali Mukhopadhyay
Jul 22, 2019·Physiological Reports·Farigol Hakem ZadehAnthony O Gramolini
Nov 24, 2017·American Journal of Physiology. Cell Physiology·Shin-Haw LeeAnthony O Gramolini
Aug 21, 2012·American Journal of Physiology. Heart and Circulatory Physiology·Roxana ChisAnthony O Gramolini
Jan 17, 2015·The Journal of Biological Chemistry·Przemek A GorskiHoward S Young

❮ Previous
Next ❯

Datasets Mentioned

BETA
EF456735

Methods Mentioned

BETA
confocal microscopy
immunoprecipitations
Co-immunoprecipitation
co-immunoprecipitations
Tandem affinity purification
transfection
immunoprecipitation

Software Mentioned

ProteinCenter
Basic Local Alignment Search Tool ( BLAST )
Basic Local Alignment Search Tool ( BLAST

Related Concepts

Related Feeds

Cardiomyopathy

Cardiomyopathy is a disease of the heart muscle, that can lead to muscular or electrical dysfunction of the heart. It is often an irreversible disease that is associated with a poor prognosis. There are different causes and classifications of cardiomyopathies. Here are the latest discoveries pertaining to this disease.