Endoplasmic reticulum stress-induced exosomal miR-27a-3p promotes immune escape in breast cancer via regulating PD-L1 expression in macrophages.

Journal of Cellular and Molecular Medicine
Xiaoli YaoXinghua Zhang

Abstract

Immune escape of breast cancer cells contributes to breast cancer pathogenesis. Tumour microenvironment stresses that disrupt protein homeostasis can produce endoplasmic reticulum (ER) stress. The miRNA-mediated translational repression of mRNAs has been extensively studied in regulating immune escape and ER stress in human cancers. In this study, we identified a novel microRNA (miR)-27a-3p and investigated its mechanistic role in promoting immune evasion. The binding affinity between miR-27a-3p and MAGI2 was predicted using bioinformatic analysis and verified by dual-luciferase reporter assay. Ectopic expression and inhibition of miR-27a-3p in breast cancer cells were achieved by transduction with mimics and inhibitors. Besides, artificial modulation of MAGI2 and PTEN was done to explore their function in ER stress and immune escape of cancer cells. Of note, exosomes were derived from cancer cells and co-cultured with macrophages for mechanistic studies. The experimental data suggested that ER stress biomarkers including GRP78, PERK, ATF6, IRE1α and PD-L1 were overexpressed in breast cancer tissues relative to paracancerous tissues. Endoplasmic reticulum stress promoted exosome secretion and elevated exosomal miR-27a-3p expres...Continue Reading

References

Sep 21, 2007·Archives of Biochemistry and Biophysics·Yali HuXiliang Zha
Apr 8, 2010·Cell·Bin-Zhi Qian, Jeffrey W Pollard
Jul 23, 2011·Genes & Cancer·Maria-Magdalena Georgescu
Feb 19, 2013·Nature Immunology·Bryan R Cullen
May 9, 2013·Annals of the New York Academy of Sciences·Maurizio Zanetti
Jan 28, 2014·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Andrew RichesSimon Powis
Nov 19, 2015·Nature·Chris Woolston
Mar 29, 2016·International Journal of Biological Sciences·Mengtao MaMinjie Wei
Jun 28, 2016·Nature Reviews. Immunology·Joep GrootjansRichard S Blumberg
Jul 8, 2016·Journal of Human Genetics·Takashi KinoshitaFei-Fei Liu
Jul 31, 2016·Journal of Cell Communication and Signaling·Arvind SubramanianSushanta K Banerjee
Mar 6, 2017·Updates in Surgery·Zoi AnastasiadiMichail Mitsis
Mar 18, 2017·The Journal of Experimental Medicine·Vikram R JunejaArlene H Sharpe
Jul 18, 2017·Radiología·J A Merino BonillaL H Ros Mendoza
Mar 9, 2018·International Journal of Cancer. Journal International Du Cancer·Yi GaoLequn Li
Mar 16, 2018·The Prostate·Stephanie N DavidGiovanna A Giannico
Mar 12, 2019·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Jiatao LiuGuoping Sun
Apr 28, 2019·Biochemical and Biophysical Research Communications·Jiwen Luo, Zheng Liu

❮ Previous
Next ❯

Citations

Sep 11, 2020·The FEBS Journal·Fernanda G Kugeratski, Raghu Kalluri
Oct 9, 2020·Technology in Cancer Research & Treatment·Jianxiang SongWencai Wang
Dec 17, 2020·OncoTargets and Therapy·Yuqi BaiTiejun Wang
Jan 9, 2021·Journal of Hematology & Oncology·Ming YiKongming Wu
Dec 4, 2020·Journal of Hematology & Oncology·Tianming ZhaoHui Zeng
Jan 14, 2021·Non-coding RNA·Heidi Schwarzenbach, Peter B Gahan
Jan 11, 2021·Seminars in Cancer Biology·Taewan Kim, Carlo M Croce
Mar 7, 2021·International Journal of Molecular Sciences·Rebecca RaueBernhard Brüne
Jun 19, 2021·Advanced Drug Delivery Reviews·Crescenzo MassaroS Rubina Baglio
Aug 10, 2021·BioMed Research International·Xiaolin RenAnhua Wu

❮ Previous
Next ❯

Methods Mentioned

BETA
biopsy
transfection
PCR
protein assay
electrophoresis
ELISA
flow cytometry
dynamic light scattering
Flow
fluorescence microscopy

Software Mentioned

Pro Plus
SPSS
ImageJ
Flowcytomix Pro
WinMDI
TargetScan
Image

Related Concepts

Related Feeds

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis