Endoplasmic Reticulum-Targeted Fluorescent Nanodot with Large Stokes Shift for Vesicular Transport Monitoring and Long-Term Bioimaging

Small
Leilei ShiXinyuan Zhu

Abstract

Herein, a highly stable aggregation-induced emission (AIE) fluorescent nanodot assembled by an amphiphilic quinoxalinone derivative-peptide conjugate, namely Quino-1-Fmoc-RACR (also termed as Q1-PEP), which exhibits large Stokes shift and an endoplasmic reticulum (ER)-targeting capacity for bioimaging is reported. It is found that the resulting nanodot can effectively enter the ER with high fluorescent emission. As the ER is mainly involved in the transport of synthesized proteins in vesicles to the Golgi or lysosomes, the Q1-PEP nanodot with ER-targeting capacity can be used to monitor vesicular transport inside the cells. Compared to conventional fluorescent dyes with small Stokes shifts, the self-assembled fluorescent nanodot shows superior resistance to photobleaching and aggregation-induced fluorescence quenching, and elimination of the spectra overlap with autofluorescence of biosubstrate owning to their AIE-active and red fluorescence emission characteristics. All these optical properties make the fluorescent nanodot suitable for noninvasive and long-term imaging both in vitro and in vivo.

References

Jul 15, 1999·Biochemical and Biophysical Research Communications·H S LiuN J Ke
Sep 21, 2002·Chemical Communications : Chem Comm·J LuoB Z Tang
Dec 3, 2002·Nature Biotechnology·Jyoti K JaiswalSanford M Simon
Dec 8, 2004·The Journal of Cell Biology·Jose Abad-RodriguezCarlos G Dotti
Apr 4, 2008·Nature·Ralph Weissleder, Mikael J Pittet
Nov 8, 2008·Accounts of Chemical Research·Elizabeth M Nolan, Stephen J Lippard
May 8, 2009·Chemical Society Reviews·Feng Wang, Xiaogang Liu
Jun 23, 2009·Advanced Drug Delivery Reviews·Sonali B FonsecaShana O Kelley
Oct 20, 2011·Chemical Society Reviews·Jing ZhouFuyou Li
Oct 25, 2011·Angewandte Chemie·Juan F AranedaRobert McDonald
Apr 11, 2012·Journal of the American Chemical Society·Daria M ShcherbakovaVladislav V Verkhusha
May 31, 2012·Journal of the American Chemical Society·Haibin ShiBin Liu
Nov 24, 2012·Nature Chemistry·Jefferson ChanChristopher J Chang
Dec 19, 2012·Journal of the American Chemical Society·Chris Wai Tung LeungBen Zhong Tang
Mar 20, 2013·Journal of the American Chemical Society·Sijie ChenBen Zhong Tang
Apr 30, 2013·Mini Reviews in Medicinal Chemistry·Cristina MaccalliniRosa Amoroso
Jun 15, 2013·Journal of the American Chemical Society·Yi LiuFuyou Li
Jul 19, 2013·Chemical Society Reviews·Xin WuYun-Bao Jiang
Feb 15, 2014·Journal of the American Chemical Society·Xumeng WuWeihong Zhu
Feb 25, 2014·ACS Nano·Dana Maria CopoloviciÜlo Langel
Apr 16, 2014·Chemical Society Reviews·Rongrong HuBen Zhong Tang
Nov 7, 2014·Chemical Society Reviews·Ryan T K KwokBen Zhong Tang
Dec 11, 2014·Journal of the American Chemical Society·I-Che WuDaniel T Chiu
Oct 23, 2015·Chemical Reviews·Ju MeiBen Zhong Tang
Aug 29, 2017·Journal of the American Chemical Society·Alexey N ButkevichStefan W Hell

❮ Previous
Next ❯

Citations

Oct 28, 2019·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Yuyao GuanZhigang Xie
Apr 29, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Sayed Mir SayedXiaolin Lu
Aug 22, 2021·Advanced Healthcare Materials·Yuqing WangXing-Jie Liang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.