Endoplasmic reticulum-to-Golgi transitions upon herpes virus infection

F1000Research
Peter WildMathias Ackermann

Abstract

Background: Herpesvirus capsids are assembled in the nucleus before they are translocated to the perinuclear space by budding, acquiring tegument and envelope, or releasing to the cytoplasm in a "naked" state via impaired nuclear envelope. One model proposes that envelopment, "de-envelopment" and "re-envelopment" are essential steps for production of infectious virus. Glycoproteins gB/gH were reported to be essential for de-envelopment, by fusion of the "primary" envelope with the outer nuclear membrane. Yet, a high proportion of enveloped virions generated from genomes with deleted gB/gH were found in the cytoplasm and extracellular space, suggesting the existence of an alternative exit route. Methods: We investigated the relatedness between the nuclear envelope and membranes of the endoplasmic reticulum and Golgi complex, in cells infected with either herpes simplex virus 1 (HSV-1) or a Us3 deletion mutant thereof, or with bovine herpesvirus 1 (BoHV-1) by transmission and scanning electron microscopy, employing freezing technique protocols that lead to improved spatial and temporal resolution. Results: Scanning electron microscopy showed the Golgi complex as a compact entity in a juxtanuclear position covered by a membrane on...Continue Reading

Datasets Mentioned

BETA
GM130

Methods Mentioned

BETA
scanning
transmission electron microscopy
Scanning Electron Microscopy
confocal microscopy
electron microscopy
electron tomography

Software Mentioned

GraphPad Prism
Huygens Essential ( SVI

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