PMID: 7539587May 1, 1995Paper

Endosomal alkalinization reduces Jmax and Km of albumin receptor-mediated endocytosis in OK cells

The American Journal of Physiology
M GekleS Silbernagl

Abstract

In this study, we investigated the effects of endosomal alkalinization on kinetics of endocytotic uptake in intact proximal tubule-derived opossum kidney cells. We used fluorescein isothiocyanate (FITC)-labeled albumin and FITC-dextran as endocytotic substrates for receptor-mediated endocytosis and fluid-phase endocytosis, respectively. The pH in endosomes labeled with either FITC-albumin or FITC-dextran rose in the presence of the vacuolar-type ATPase inhibitor, bafilomycin A1, and in the presence of NH4Cl. Cytoplasmic pH, decreased in the presence of bafilomycin A1, but was not significantly different from control during prolonged exposure of the cells to NH4Cl. Endocytotic uptake of FITC-dextran was not affected by endosomal pH changes. Endocytotic uptake of FITC-albumin was reduced markedly by bafilomycin A1 (decrease of maximum transport rate and apparent affinity). Selective alkalinization of endosomes using NH4Cl (i.e., with the cytoplasmic pH not different from control) reduced FITC-albumin uptake in a similar way but to a lesser extent than did bafilomycin A1. Intracellular albumin degradation was impaired by bafilomycin A1 and NH4Cl. Prevention of endosome-lysosome fusion (lowering the temperature to 20 degrees C) abo...Continue Reading

Citations

Jan 15, 2002·American Journal of Physiology. Renal Physiology·Noriyoshi KobayashiYasuhiko Tomino
Aug 9, 2002·American Journal of Physiology. Renal Physiology·Michael GekleErik I Christensen
Dec 12, 2002·American Journal of Physiology. Cell Physiology·Corinne Isnard-BagnisSylvie Breton
Jul 19, 2008·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·M CasartelliB Giordana
Oct 21, 2011·Biotechnology and Bioengineering·Nicholas FerrellWilliam H Fissell
Feb 16, 2005·Annual Review of Physiology·Michael Gekle
May 27, 2004·American Journal of Physiology. Renal Physiology·Phuong-Chi PhamAdam M Sun
Jun 28, 2018·American Journal of Physiology. Renal Physiology·Mark A BryniarskiMarilyn E Morris
Sep 24, 2004·Physiological Reviews·Carsten A WagnerJohn P Geibel
Jun 8, 2001·News in Physiological Sciences : an International Journal of Physiology Produced Jointly by the International Union of Physiological Sciences and the American Physiological Society·Michael Gekle
Apr 29, 2004·American Journal of Physiology. Renal Physiology·Michael GekleMartin Wiemann
May 31, 2018·Physiological Reviews·Thomas J Jentsch, Michael Pusch
Apr 15, 1996·Brain Research·G Schrader-Fischer, P A Paganetti
Jun 3, 2021·International Journal of Molecular Sciences·Jakub GburekKrzysztof Gołąb

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