Endosomal escape efficiency of fusogenic B18 and B55 peptides fused with anti-EGFR single chain Fv as estimated by nuclear translocation

Journal of Biochemistry
Keisuke NiikuraNobuhide Doi

Abstract

Although monoclonal antibodies have been used not only as analytical tools but also as biologic therapeutics, they cannot target intracellular proteins due to their large molecular size and low membrane permeability, which limit their applications. During previous attempts to delivery antibodies intracellularly, the low efficiency of escape from endosomes to the cytosol reduced the bioavailability of antibodies or antibody-conjugated effectors. Recently, we found that the fusogenic peptides (FPs) B18 and B55 from bindin, a sea urchin gamete recognition protein, facilitated the endosomal escape of FP-fused enhanced green fluorescent protein (eGFP) and/or of co-administered cargos such as dextrans [Niikura et al. A fusogenic peptide from a sea urchin fertilization protein promotes intracellular delivery of biomacromolecules by facilitating endosomal escape. J. Release 2015;212:85-93]. In this study, we constructed FP-fused anti-epidermal growth factor receptor (EGFR) single-chain Fv (αEGFR[scFv]) proteins and evaluated their endosomal escape efficiency by utilizing a nuclear localization signal). When the FP-fused αEGFR[scFv] proteins were incubated with A431 cells, the estimated endosomal escape efficiency of αEGFR[scFv]-B18 was...Continue Reading

References

Sep 15, 2000·Journal of Immunological Methods·N Baumgarth, M Roederer
Mar 1, 2003·Journal of Immunological Methods·Biji T Kurien, R Hal Scofield
Oct 16, 2004·Biochemical and Biophysical Research Communications·Junya IchinoseYasushi Sako
Oct 3, 2006·Nucleic Acids Research·Isao FukudaHiroshi Yanagawa
Aug 1, 2007·Allergologia et immunopathologia·M A Martin-Mateos
Apr 29, 2008·Biochimica Et Biophysica Acta·Eric VivèsAndré Pèlegrin
Apr 2, 2009·Nucleic Acids Research·Noriko TabataHiroshi Yanagawa
Jan 23, 2010·MAbs·Aaron L Nelson
Aug 3, 2010·Biochemical and Biophysical Research Communications·Ying-Nai WangMien-Chie Hung
Sep 21, 2010·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Richard W Burry
Feb 2, 2012·The Journal of Biological Chemistry·Javier Chaparro-RiggersArvind Rajpal
Nov 22, 2012·Proceedings of the National Academy of Sciences of the United States of America·Justin L LorieauAdriaan Bax
Jan 9, 2013·Biology of the Cell·Sheng-Jun XiaoAkira Inoue
Aug 21, 2013·The Journal of Investigative Dermatology·Stephanie D Gan, Kruti R Patel
Sep 17, 2013·Journal of Virological Methods·Aninthita PhalapholWanpen Chaicumpa
Jan 1, 2012·Pharmaceuticals·Alfredo Erazo-OliverasJean-Philippe Pellois
Dec 3, 2013·Journal of Controlled Release : Official Journal of the Controlled Release Society·Feihu WangFang Jin
Mar 1, 2014·Blood·Alan S WayneIra Pastan
Jun 3, 2014·Biochimica Et Biophysica Acta·Jing Zhang, Terence H Rabbitts
Aug 13, 2014·Journal of Peptide Science : an Official Publication of the European Peptide Society·Siegmund Reissmann
Jun 21, 2015·Journal of Controlled Release : Official Journal of the Controlled Release Society·Keisuke NiikuraNobuhide Doi

❮ Previous
Next ❯

Citations

Apr 8, 2017·Journal of Controlled Release : Official Journal of the Controlled Release Society·Kei SudoNobuhide Doi
Jun 12, 2020·Expert Opinion on Drug Delivery·Suchada NiamsuphapChristopher B Howard
Aug 3, 2018·Nature Communications·Duverney Chaverra-RodriguezJason L Rasgon
Feb 23, 2021·Journal of Peptide Science : an Official Publication of the European Peptide Society·Siegmund Reissmann, Margarita P Filatova
Sep 10, 2021·Proceedings of the National Academy of Sciences of the United States of America·Victor D Vacquier

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Journal of Controlled Release : Official Journal of the Controlled Release Society
Keisuke NiikuraNobuhide Doi
Current Topics in Developmental Biology
K Ohlendieck, W J Lennarz
Journal of Controlled Release : Official Journal of the Controlled Release Society
Shu YangRodney F Minchin
Journal of Controlled Release : Official Journal of the Controlled Release Society
Yan MaYu-Hui Zhang
© 2021 Meta ULC. All rights reserved