Endosperm-specific expression of serotonin N-hydroxycinnamoyltransferase in rice.

Plant Foods for Human Nutrition
Kyungjin LeeKyoungwhan Back

Abstract

Serotonin N-hydroxycinnamoyltransferase (SHT) is a key enzyme in the synthesis of feruloylserotonin (FS) and 4-coumaroylserotonin (CS). These serotonin derivatives show strong antioxidant activity, making them valuable for both nutritional and pharmacological use in humans. Ectopic expression of SHT under the control of the endosperm specific-glutelin and prolamin promoters from rice was produced via Agrobacterium-mediated transformation. SHT expression was confirmed by Southern blot analysis, followed by Northern blotting and SHT enzyme activity analyses using total RNA and protein, respectively, extracted from transgenic seeds. The glutelin A3 (GluA3) promoter produced low SHT mRNA expression in rice seeds, whereas the prolamin promoter expressed high levels of SHT mRNA. In spite of the ectopic expression of SHT in rice seeds, both transgenic genotypes accumulated levels of serotonin derivatives similar to those found in wild-type rice. Furthermore, our data suggest that serotonin, rather than phenylpropanid-CoAs, is the rate-limiting substrate in the biosynthesis of serotonin derivatives in SHT-overexpressing transgenic rice seeds.

References

Dec 20, 1999·Journal of Agricultural and Food Chemistry·M Watanabe
Dec 29, 2000·Bioscience, Biotechnology, and Biochemistry·T NiwaY Shimizu
Mar 26, 2002·Journal of Experimental Botany·Peter R Shewry, Nigel G Halford
Oct 17, 2003·Phytochemistry·Eiji TanakaMitsuya Tsuda

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Citations

Mar 25, 2009·Applied Microbiology and Biotechnology·Kiyoon KangKyoungwhan Back
Jul 7, 2009·Biotechnology Advances·Arun K Sharma, Manoj K Sharma
Jun 12, 2010·Journal of Pineal Research·Susan J MurchPraveen K Saxena
Nov 17, 2009·Journal of Animal Physiology and Animal Nutrition·S Ragnarsson, J E Lindberg
Nov 9, 2021·Frontiers in Plant Science·René LemckeMichael F Lyngkjær

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