Endosperm sugar accumulation caused by mutation of PHS8/ISA1 leads to pre-harvest sprouting in rice

The Plant Journal : for Cell and Molecular Biology
Lin DuChengcai Chu

Abstract

Pre-harvest sprouting (PHS) is an unfavorable trait in cereal crops that could seriously decrease grain yield and quality. Although some PHS-associated quantitative trait loci or genes in cereals have been reported, the molecular mechanism underlying PHS remains largely elusive. Here, we characterized a rice mutant, phs8, which exhibits PHS phenotype accompanied by sugary endosperm. Map-based cloning revealed that PHS8 encodes a starch debranching enzyme named isoamylase1. Mutation in PHS8 resulted in the phytoglycogen breakdown and sugar accumulation in the endosperm. Intriguingly, with increase of sugar contents, decreased expression of OsABI3 and OsABI5 as well as reduced sensitivity to abscisic acid (ABA) were found in the phs8 mutant. Using rice suspension cell system, we confirmed that exogenous sugar is sufficient to suppress the expression of both OsABI3 and OsABI5. Furthermore, overexpression of OsABI3 or OsABI5 could partially rescue the PHS phenotype of phs8. Therefore, our study presents important evidence supporting that endosperm sugar not only acts as an essential energy source for seed germination but also determines seed dormancy and germination by affecting ABA signaling.

References

Oct 1, 1992·The Plant Cell·J GiraudatH M Goodman
Mar 1, 1994·Plant Molecular Biology·T HattoriS T Hamasuna
Oct 3, 1999·The Plant Journal : for Cell and Molecular Biology·U HoeckerD R McCarty
Dec 28, 1999·Proceedings of the National Academy of Sciences of the United States of America·T HoboT Hattori
Dec 15, 2000·Plant Physiology·S I Gibson
Feb 24, 2001·The Plant Journal : for Cell and Molecular Biology·S MerlotJ Giraudat
Sep 27, 2001·Proceedings of the National Academy of Sciences of the United States of America·S L StoneJ J Harada
Jun 5, 2002·The Plant Cell·Filip RollandJen Sheen
Jul 9, 2002·The Plant Journal : for Cell and Molecular Biology·Rachel A BurtonKay Denyer
Nov 2, 2002·The Plant Journal : for Cell and Molecular Biology·Luis Lopez-MolinaNam-Hai Chua
Jul 2, 2003·Genome Génome / Conseil National De Recherches Canada·S RahmanM Morell
Nov 6, 2003·The Plant Cell·Gregory N Harrington, Daniel R Bush
Dec 17, 2003·The Plant Journal : for Cell and Molecular Biology·Yuichiro TsuchiyaPeter McCourt
Jan 22, 2005·Plant & Cell Physiology·Jun-Ichi ItohYasuo Nagato
Mar 2, 2006·The Plant Journal : for Cell and Molecular Biology·Anthony A MillarFrank Gubler
May 4, 2006·Annual Review of Plant Biology·Filip RollandJen Sheen
Nov 4, 2006·Plant, Cell & Environment·Fred RookMichael W Bevan
Dec 28, 2006·Cell·John F DoebleyBruce D Smith
Jan 23, 2008·The Plant Journal : for Cell and Molecular Biology·Jun FangChengcai Chu
Feb 9, 2008·Annual Review of Plant Biology·Ruth FinkelsteinCamille Steber
Mar 28, 2008·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·F C OgbonnayaJ M Winchester
Aug 23, 2008·Proceedings of the National Academy of Sciences of the United States of America·Kenji FujinoMasahiro Yano
May 19, 2009·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Yimian MaChengcai Chu
Jun 11, 2009·Plant Signaling & Behavior·Jun Fang, Chengcai Chu
Aug 12, 2009·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Jesse D MunkvoldMark E Sorrells
Mar 3, 2010·Annual Review of Plant Biology·Samuel C ZeemanAlison M Smith
Mar 3, 2010·Annual Review of Plant Biology·Sean R CutlerSuzanne R Abrams
Mar 12, 2010·Proceedings of the National Academy of Sciences of the United States of America·Kazuhiko SugimotoMasahiro Yano

❮ Previous
Next ❯

Citations

Feb 23, 2020·International Journal of Molecular Sciences·Shaowen YuanSibin Yu
Jul 10, 2020·Journal of Experimental Botany·Rosa L López-MarquésMichael Palmgren
Aug 8, 2020·Plant & Cell Physiology·Shaopei Gao, Chengcai Chu
Aug 23, 2019·The Plant Journal : for Cell and Molecular Biology·Fan XuChengcai Chu
Jan 12, 2021·Frontiers in Plant Science·Lu AnZhenghui Liu
Jan 21, 2021·Journal of Experimental Botany·Li TaiKun-Ming Chen
Jul 28, 2021·The Plant Journal : for Cell and Molecular Biology·Qin ZhangJean-Philippe F Ral

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.