Endostatin gene therapy delivered by attenuated Salmonella typhimurium in murine tumor models

Cancer Gene Therapy
Kang LiangQingke Kong

Abstract

Salmonella typhimurium (hereafter S. typhimurium), as Gram-negative facultative anaerobic bacteria, are good candidates for cancer therapy and delivering therapeutic antitumor agents. However, it is necessary to reduce the virulence of such bacteria and enhance their tumor-targeting ability, and their immunostimulatory ability to induce tumor cell apoptosis. In this study, we constructed a S. typhimurium mutant named S634 harboring aroA mutation and additional mutations involved in modifications of lipid A. Upon intraperitoneal infection in mice, the aroA-deficient strain S634 showed greatly attenuated virulence and preferential accumulation within tumor tissue. We next investigated the ability of S636, the asd mutant derivative of S634, to deliver the anti-angiogenic agent "endostatin" (S636/pES) and to inhibit tumor growth in mouse CT26 colon carcinoma and B16F10 melanoma models. S636/pES-treated tumor-bearing mice showed suppressed tumor growth and prolonged survival, compared to mice treated with either the bacteria carrying empty plasmids or PBS intraperitoneally. Immunohistochemical studies demonstrated that, when tumor-bearing mice were infected with S636/pES, Salmonella colonization and endostatin expression were accomp...Continue Reading

References

Jan 3, 1991·The New England Journal of Medicine·N WeidnerJ Folkman
Apr 14, 1999·Cell Death and Differentiation·A G Porter, R U Jänicke
Apr 9, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·M Shichiri, Y Hirata
Jan 5, 2002·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·John F TosoSteven A Rosenberg
Jan 30, 2002·The Journal of Biological Chemistry·Jun-ichi HanaiVikas P Sukhatme
May 25, 2002·The Journal of Biological Chemistry·Young-Mi KimYoung-Guen Kwon
Jun 5, 2002·Annual Review of Biochemistry·Christian R H Raetz, Chris Whitfield
Apr 17, 2003·Proceedings of the National Academy of Sciences of the United States of America·Akulapalli SudhakarRaghu Kalluri
Mar 31, 2004·Trends in Microbiology·Kensuke Miyake
Jan 13, 2005·Proceedings of the National Academy of Sciences of the United States of America·Ming ZhaoRobert M Hoffman
May 4, 2005·Cancer Research·Francesca AvogadriMaria Rescigno
Sep 24, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Athmane TeghanemtTheresa L Gioannini
Oct 18, 2005·Protein Expression and Purification·Lynn E BretscherCandice S Klug
Aug 1, 2006·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Matthew H KulkeCharles S Fuchs
Mar 17, 2007·Annual Review of Biochemistry·Christian R H RaetzRussell E Bishop
Apr 24, 2007·International Journal of Medical Microbiology : IJMM·Jochen StritzkerAladar A Szalay
Dec 9, 2008·Trends in Immunology·Steven G ReedMartin Friede
Feb 10, 2009·Journal of Cellular Biochemistry·Katsuhiro HayashiRobert M Hoffman
Jun 16, 2010·International Journal of Medical Microbiology : IJMM·Jochen StritzkerAladar A Szalay
Oct 15, 2010·Nature Reviews. Cancer·Neil S Forbes
Apr 19, 2011·Current Opinion in Biotechnology·Robert M Hoffman
Jun 3, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Qingke KongRoy Curtiss
Dec 24, 2011·Integrative Biology : Quantitative Biosciences From Nano to Macro·Bhushan J Toley, Neil S Forbes
Apr 26, 2012·Proceedings of the National Academy of Sciences of the United States of America·Umeharu OhtoToshiyuki Shimizu

❮ Previous
Next ❯

Citations

Dec 13, 2019·Experimental & Molecular Medicine·Mai Thi-Quynh DuongJung-Joon Min
May 28, 2020·International Journal of Molecular Sciences·Eva AndreuzziMaurizio Mongiat
Oct 11, 2020·Biotechnology and Bioengineering·Kang LiangQingke Kong
Mar 20, 2021·Frontiers in Oncology·Fereshteh BadieHamed Mirzaei
Dec 12, 2020·Trends in Cancer·Brendan Fu-Long SieowMatthew Wook Chang
May 13, 2021·Seminars in Cancer Biology·Deepti DiwanVijai Kumar Gupta

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

Themofisher
SPSS
Image
Pro Plus

Related Concepts

Related Feeds

Angiogenesis Inhibitors to Treat Cancer

Cancer treatments including angiogenesis inhibitors prevent tumor cells from receiving nutrients and oxygen. Here is the latest research on angiogenesis inhibitors for the treatment of cancer.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.