Endostatin gene therapy for liver cancer by a recombinant adenovirus delivery

World Journal of Gastroenterology : WJG
Li LiWenlin Huang

Abstract

To investigate the expression of adenovirus-mediated human endostatin (Ad/hEndo) gene transfer and its effect on the growth of hepatocellular carcinoma (HCC) BEL-7402 xenografted tumors. Immunohistochemistry analysis with an anti-endostatin antibody was preformed to detect endostatin protein expression in HCC BEL-7402 cells infected with Ad/hEndo. MTT assay was used to investigate the effects of Ad/hEndo on proliferation of human umbilical vein endothelial cells (HUVEC). Intra-tumoral injections of 1X10(9) pfu Ad/hEndo was given to treat BEL-7402 xenografted tumors in nude mice once weekly for 6 wk. Mice received injections of Ad/LacZ and DMEM were regarded as control groups. After intra-tumoral administration with Ad/hEndo, the endostatin mRNA expression in tumor tissue was analyzed by Northern blotting, and plasma endostatin levels were determined using enzyme-linked immunosorbent assay (ELISA). High level expression of endostatin gene was detected in the infected HCC BEL-7402 cells. Ad/hEndo significantly inhibited HUVEC cell proliferation by 57.2% at a multiplicity of infection (MOI) of 20. After 6-week treatment with Ad/hEndo, the growth of treated tumors was inhibited by 46.50% compared to the Ad/LacZ control group (t=2.7...Continue Reading

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Citations

Nov 1, 2015·Advanced Drug Delivery Reviews·Chiara PoluzziLiliana Schaefer
Jan 3, 2012·Digestive and Liver Disease : Official Journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver·Yan-Chun ZhouQing-Xia Niu
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Mar 26, 2014·Molecular Therapy : the Journal of the American Society of Gene Therapy·Wen YeWenlin Huang
Feb 19, 2010·Human Gene Therapy·Haritha SamaranayakeSeppo Ylä-Herttuala
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Dec 31, 2017·Survey of Ophthalmology·Wei ZhongDimitri T Azar

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