Endosymbiont interference and microbial diversity of the Pacific coast tick, Dermacentor occidentalis, in San Diego County, California

PeerJ
Nikos GurfieldScott T Kelley

Abstract

The Pacific coast tick, Dermacentor occidentalis Marx, is found throughout California and can harbor agents that cause human diseases such as anaplasmosis, ehrlichiosis, tularemia, Rocky Mountain spotted fever and rickettsiosis 364D. Previous studies have demonstrated that nonpathogenic endosymbiotic bacteria can interfere with Rickettsia co-infections in other tick species. We hypothesized that within D. occidentalis ticks, interference may exist between different nonpathogenic endosymbiotic or nonendosymbiotic bacteria and Spotted Fever group Rickettsia (SFGR). Using PCR amplification and sequencing of the rompA gene and intergenic region we identified a cohort of SFGR-infected and non-infected D. occidentalis ticks collected from San Diego County. We then amplified a partial segment of the 16S rRNA gene and used next-generation sequencing to elucidate the microbiomes and levels of co-infection in the ticks. The SFGR R. philipii str. 364D and R. rhipicephali were detected in 2.3% and 8.2% of the ticks, respectively, via rompA sequencing. Interestingly, next generation sequencing revealed an inverse relationship between the number of Francisella-like endosymbiont (FLE) 16S rRNA sequences and Rickettsia 16S rRNA sequences withi...Continue Reading

References

Jan 1, 1975·Annals of the New York Academy of Sciences·W Burgdorfer, L P Brinton
Nov 1, 1973·Journal of Invertebrate Pathology·W BurgdorferL E Hughes
May 1, 1981·The American Journal of Tropical Medicine and Hygiene·R N PhilipE A Casper
Jan 1, 1981·The American Journal of Tropical Medicine and Hygiene·R S LaneB C Nelson
Apr 1, 1997·International Journal of Systematic Bacteriology·M L NiebylskiT G Schwan
Jun 11, 1998·Emerging Infectious Diseases·A F Azad, C B Beard
Jan 30, 1999·Applied and Environmental Microbiology·M L NiebylskiT G Schwan
Dec 10, 2003·Journal of Clinical Microbiology·Jessica L VersageJeannine M Petersen
Jun 10, 2004·Journal of Medical Entomology·Glen A Scoles
Mar 12, 2005·BMC Genetics·Jeffrey L JensenScott T Kelley
Nov 5, 2005·Applied and Environmental Microbiology·Kiersten J KugelerJeannine M Petersen
Jul 18, 2006·Nucleic Acids Research·T Z DeSantisG L Andersen
Nov 23, 2006·Annals of the New York Academy of Sciences·Marina E EremeevaGregory A Dasch
Jun 8, 2007·Journal of Clinical Microbiology·Sandor E KarpathyMarina E Eremeeva
Jun 7, 2008·Journal of Medical Entomology·Mary E WikswoMarina E Eremeeva
Aug 25, 2009·International Journal of Systematic and Evolutionary Microbiology·David Miñana-GalbisRosa M Oliart-Ros
Aug 28, 2009·Seminars in Reproductive Medicine·Carter M Owen, James H Segars
Oct 30, 2009·The ISME Journal·Ryan T JonesAndrew P Martin
Nov 17, 2009·Bioinformatics·J Gregory CaporasoRob Knight
Jan 16, 2010·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Marc R ShapiroMarina E Eremeeva
Mar 13, 2010·PloS One·Morgan N PriceAdam P Arkin
Apr 13, 2010·Nature Methods·J Gregory CaporasoRob Knight
Aug 17, 2010·Bioinformatics·Robert C Edgar
Jul 19, 2011·Nature Methods·Dan KnightsScott T Kelley
Jun 12, 2012·Journal of Medical Entomology·Ryan T JonesKenneth L Gage
Feb 2, 2013·PloS One·Krissi M HewittScott T Kelley
Mar 22, 2013·Genome Biology·Scott T Kelley, Jack A Gilbert
Apr 19, 2013·The Pediatric Infectious Disease Journal·Samantha H JohnstonSharon L Messenger
Oct 29, 2013·Applied and Environmental Microbiology·Loganathan PonnusamySteven R Meshnick
Mar 13, 2014·Journal of Medical Entomology·Khemraj BudachetriShahid Karim
Aug 26, 2014·Ticks and Tick-borne Diseases·Xue-Chao ZhangHai-Jun Xu
May 6, 2015·Trends in Parasitology·Sukanya Narasimhan, Erol Fikrig
Jul 8, 2015·Applied and Environmental Microbiology·Will Van TreurenSteven R Meshnick

❮ Previous
Next ❯

Citations

May 27, 2017·Parasites & Vectors·Rongrong WangJingze Liu
Jul 10, 2018·Journal of Medical Entomology·Christopher D PaddockKerry A Padgett
Oct 31, 2019·Parasites & Vectors·Troy J KieranNicole L Gottdenker
Jan 6, 2018·Parasites & Vectors·Telleasha L GreayPeter J Irwin
Apr 24, 2020·Journal of Medical Entomology·Cameron J OsborneTricia A Van Laar
May 30, 2019·Frontiers in Cellular and Infection Microbiology·Briana Zellner, Jason F Huntley
Dec 15, 2020·Parasite Immunology·Sarah Irène Bonnet, Thomas Pollet
Jan 9, 2021·Ticks and Tick-borne Diseases·Sandra Díaz-SánchezJosé de la Fuente
Nov 15, 2020·Applied and Environmental Microbiology·Jonathan D OliverUlrike G Munderloh
Feb 14, 2020·Ticks and Tick-borne Diseases·Siobhon L EganCharlotte L Oskam
Jun 10, 2021·PLoS Neglected Tropical Diseases·Nayyereh ChoubdarMohammad Ali Oshaghi
Jun 16, 2021·Ticks and Tick-borne Diseases·Leta EliasArtem S Rogovskyy

❮ Previous
Next ❯

Datasets Mentioned

BETA
CP013133.1
CP003342.1
U11019.1
CP006010.1
CP003311.1
AY805304.1
AY375402.1
GU968868.1
FJ468434.1
and

Methods Mentioned

BETA
PCR
Illumina sequencing
electrophoresis
Illumina

Software Mentioned

Analysis of Similarity ( ANOSIM )
SourceTracker
R studio
R
PyNAST
BLASTn
QIIME ( Quantitative Insights Into Microbial Ecology )
vegan
PERMANOVA
UCLUST

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America
Marc R ShapiroMarina E Eremeeva
Ticks and Tick-borne Diseases
Alexandra SzigetiMiklós Gyuranecz
© 2022 Meta ULC. All rights reserved