Endothelial activation, haemostasis and thrombosis biomarkers in Ugandan children with severe malaria participating in a clinical trial

Malaria Journal
Susan M GrahamW Conrad Liles

Abstract

Malaria is a major cause of morbidity and mortality in sub-Saharan Africa, and poor outcomes have been associated with endothelial activation. In this study, biomarkers of endothelial activation, haemostasis, and thrombosis were measured in Ugandan children with severe malaria who participated in a clinical trial, in order to investigate associations between these processes. Serum and plasma were collected from participants at baseline (day 1), and on days 2, 3, 4, and 14. Von Willebrand factor (VWF) antigen was measured in stored plasma samples from all trial participants, and its association with mortality and changes over time were analysed. VWF multimer patterns were evaluated in baseline serum samples by gel electrophoresis followed by Western blotting. Levels of angiopoietins 1 and 2, VWF antigen, total active VWF, ADAMTS13, platelet counts, apolipoprotein A1, and syndecan-1 were measured in stored serum samples from 12 survivors at baseline and day 4. VWF antigen levels were associated with mortality, and decreased over time in survivors. Baseline VWF antigen and total active VWF levels were elevated, and very large multimers were present in the baseline serum of several patients. Higher platelet counts were associated w...Continue Reading

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Citations

Feb 18, 2020·Virulence·Harsita PatelAubrey J Cunnington
Jun 3, 2020·Journal of Thrombosis and Haemostasis : JTH·Sirima KraisinSimon F De Meyer
May 18, 2019·Journal of Thrombosis and Haemostasis : JTH·Sirima KraisinSimon F De Meyer
Aug 18, 2021·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Margaret A BushJoe Brice Weinberg

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Methods Mentioned

BETA
light microscopy
electrophoresis
ELISA

Clinical Trials Mentioned

NCT01255215

Software Mentioned

ImageQuant TL
Stata

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