Endothelial alterations during inhaled NO in lambs with pulmonary hypertension: implications for rebound hypertension

American Journal of Physiology. Lung Cellular and Molecular Physiology
Gregory A RossJeffrey R Fineman

Abstract

Clinically significant increases in pulmonary vascular resistance (PVR) have been noted upon acute withdrawal of inhaled nitric oxide (iNO). Previous studies in the normal pulmonary circulation demonstrate that iNO increases endothelin-1 (ET-1) levels and decreases endogenous nitric oxide synthase (NOS) activity, implicating an endothelial etiology for the increase in resistance upon iNO withdrawal. However, the effect of iNO on endogenous endothelial function in the clinically relevant pulmonary hypertensive circulation is unknown. The objective of this study was to determine the effects of iNO on endogenous NO-cGMP and ET-1 signaling in lambs with preexisting pulmonary hypertension secondary to increased pulmonary blood flow. Eight fetal lambs underwent in utero placement of an aortopulmonary vascular graft (shunt lambs). After delivery (4 wk), the shunt lambs were mechanically ventilated with iNO (40 ppm) for 24 h. After 24 h of inhaled NO, plasma ET-1 levels increased by 34.8% independently of changes in protein levels (P < 0.05). Contrary to findings in normal lambs, total NOS activity did not decrease during iNO. In fact, Western blot analysis demonstrated that tissue endothelial NOS protein levels decreased by 43% such t...Continue Reading

References

Oct 16, 1989·Biochemical and Biophysical Research Communications·Y T XuanW D Watkins
Apr 11, 1995·The American Journal of Physiology·R H SteinhornF C Morin
Mar 30, 1994·Biochemical and Biophysical Research Communications·T FukurodaM Nishikibe
Jan 26, 1996·Biochemical Pharmacology·A J Turner, L J Murphy
Dec 1, 1996·The Annals of Thoracic Surgery·A M AtzD L Wessel
Feb 27, 1997·The New England Journal of Medicine·UNKNOWN Neonatal Inhaled Nitric Oxide Study Group
Jun 5, 1998·The American Journal of Physiology·A M SheehyS M Black
Nov 14, 1998·The American Journal of Physiology·S M BlackS J Soifer
Oct 28, 1999·American Journal of Respiratory Cell and Molecular Biology·I S YuhannaP W Shaul
Feb 26, 2000·Cardiovascular Research·A PapapetropoulosW C Sessa
Dec 21, 2000·American Journal of Physiology. Heart and Circulatory Physiology·R H SteinhornJ R Fineman
Feb 13, 2001·American Journal of Physiology. Heart and Circulatory Physiology·D M McMullanJ R Fineman
Feb 24, 2001·American Journal of Physiology. Renal Physiology·R Govers, T J Rabelink
Apr 11, 2001·Cardiology in the Young·M Beghetti, I Adatia
Oct 13, 2001·American Journal of Physiology. Lung Cellular and Molecular Physiology·S M BlackJ R Fineman
Feb 5, 2002·Annual Review of Physiology·Philip W Shaul
Apr 30, 2004·The Journal of Thoracic and Cardiovascular Surgery·Stephan ThelitzJeffrey R Fineman

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Citations

May 19, 2009·American Journal of Physiology. Lung Cellular and Molecular Physiology·Sanjiv KumarStephen M Black
Mar 28, 2006·Proceedings of the American Thoracic Society·Joan B Mannick
Nov 26, 2015·Interactive Cardiovascular and Thoracic Surgery·Ismihan Selen OnanErsin Erek
Dec 20, 2007·Journal of Cardiovascular Pharmacology·Marc Iglarz, Martine Clozel
Nov 1, 2005·American Journal of Physiology. Lung Cellular and Molecular Physiology·Peter OishiJeffrey R Fineman
Sep 11, 2007·American Journal of Physiology. Lung Cellular and Molecular Physiology·Louis G ChicoineBenjimen R Walker
Jul 16, 2008·Jornal brasileiro de pneumologia : publicaça̋o oficial da Sociedade Brasileira de Pneumologia e Tisilogia·Carlos Alan Dias-JuniorJosé Eduardo Tanus-Santos

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