Endothelial apoptosis and circulating endothelial cells after bypass grafting with and without cardiopulmonary bypass

European Journal of Cardio-thoracic Surgery : Official Journal of the European Association for Cardio-thoracic Surgery
Franz-Xaver SchmidDietrich E Birnbaum

Abstract

We compared profiles of the numbers of circulating endothelial cells (CEC) and the apoptosis-inducing capacity of serum samples on human endothelial cells (hEC) in on-pump and off-pump coronary artery bypass grafting (CABG) patients. Blood samples from 30 patients undergoing CABG (randomly assigned to two groups: 15 patients off-pump and 15 on-pump (cardiopulmonary bypass, CPB)) were collected after induction of anesthesia (preoperatively), at weaning from CPB/end of bypass grafting (0 h), and 1, 6, 12, 24, and 48 h afterwards. CEC were isolated with immunomagnetic anti-CD146-coated Dynabeads, and counted in a Nageotte chamber. The apoptosis-inducing activity of serum samples on hEC was examined by a tissue culture assay system. Apoptotic and normal cells were identified using phase contrast/fluorescence microscopy after DNA dye staining. CEC numbers and proportions of apoptotic hEC were significantly elevated during and after surgery in both groups (p<0.01). Compared with the on-pump group, CEC and proportions of apoptotic hEC were significantly lower (p=0.04 and p=0.03, respectively) in patients having CABG performed off-pump. Starting at comparable baseline levels, the mean CEC-number was highest at 6h postoperatively with 8...Continue Reading

Citations

May 10, 2007·Scandinavian Cardiovascular Journal : SCJ·Pavel KunesJan Krejsek
Dec 25, 2015·Journal of Cardiovascular and Thoracic Research·Saddiq Mohammad QaziMartin Agge Nørgaard
Sep 16, 2015·Innovations : Technology and Techniques in Cardiothoracic and Vascular Surgery·John D PuskasVipin Zamvar
Mar 16, 2012·The Cochrane Database of Systematic Reviews·Christian H MøllerChristian Gluud
Sep 30, 2015·Journal of Cardiovascular Medicine·Alessandro ParolariElena Tremoli
Nov 18, 2015·Molecular Medicine Reports·Yun-Jiang ZhengShu-Ming Pan

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