Endothelial cell adhesion on RGD-containing methacrylate terpolymers

Journal of Biomedical Materials Research. Part a
Garland W Fussell, Stuart L Cooper

Abstract

Hexyl methacrylate (HMA), methyl methacrylate (MMA), and methacrylic acid (MAA) were used as comonomers to produce a low glass transition temperature material, potentially useful in fabricating a small diameter vascular graft. Because it has been shown that grafts seeded with endothelial cells have better resistance to thrombosis, RGD-based peptide sequences were incorporated into the terpolymer. The two methods used for incorporating peptide sequences were a chain transfer reaction during polymerization, and a coupling reaction between the amine terminus of the peptide and the carboxyl groups of the MAA. Polymers were synthesized using the chain transfer reaction with peptide concentrations ranging from 1.7 to 7.0 micromol/g. Weight-average molecular weights decreased with increasing peptide concentration from 310,000 g/mol for the terpolymer without peptide, to 110,000 g/mol for a peptide concentration of 7.0 micromol/g. As a result, Young's modulus decreased with increasing peptide concentration. Terpolymers with peptides attached through a coupling reaction showed no decrease in molecular weight or mechanical properties. Confocal microscopy showed cells seeded on the RGD surfaces adhered and spread, while terpolymers with R...Continue Reading

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Citations

Apr 17, 2012·Acta Biomaterialia·Daniel E Heath, Stuart L Cooper
Sep 2, 2006·Journal of Biomedical Materials Research. Part a·Bin LiJames H-C Wang
Jan 13, 2012·Journal of Biomedical Materials Research. Part a·Xin WangStuart L Cooper
Aug 10, 2010·Journal of Biomedical Materials Research. Part a·Daniel E Heath, Stuart L Cooper
Aug 10, 2010·Journal of Biomedical Materials Research. Part a·Daniel E HeathStuart L Cooper
Mar 29, 2014·Journal of Tissue Engineering and Regenerative Medicine·Ana Rubina PerestreloJosé António Belo
Sep 30, 2010·Journal of Tissue Engineering and Regenerative Medicine·Jose F Alvarez-BarretoVassilios I Sikavitsas
Jun 2, 2009·Journal of Biomedical Materials Research. Part a·Menno L W Knetsch, Leo H Koole
Jan 17, 2009·Journal of Biomedical Materials Research. Part a·A N VelevaS L Cooper
Nov 21, 2009·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Daniel E Heath, Stuart L Cooper
Jun 8, 2006·Journal of Biomedical Materials Research. Part a·Nicholas S LudwigKhalid N Kader
Jul 20, 2006·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Esra CerAli Tuncel
Apr 26, 2007·Macromolecular Bioscience·Jose F Alvarez-Barreto, Vassilios I Sikavitsas
Jun 1, 2005·Journal of Biomedical Materials Research. Part a·Anka N VelevaStuart L Cooper
Aug 2, 2017·Chemical Reviews·Samuel Clark LigonRolf Mülhaupt

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