Endothelial Cell Bioenergetics and Mitochondrial DNA Damage Differ in Humans Having African or West Eurasian Maternal Ancestry

Circulation. Cardiovascular Genetics
David M KrzywanskiScott W Ballinger

Abstract

We hypothesized that endothelial cells having distinct mitochondrial genetic backgrounds would show variation in mitochondrial function and oxidative stress markers concordant with known differential cardiovascular disease susceptibilities. To test this hypothesis, mitochondrial bioenergetics were determined in endothelial cells from healthy individuals with African versus European maternal ancestries. Bioenergetics and mitochondrial DNA (mtDNA) damage were assessed in single-donor human umbilical vein endothelial cells belonging to mtDNA haplogroups H and L, representing West Eurasian and African maternal ancestries, respectively. Human umbilical vein endothelial cells from haplogroup L used less oxygen for ATP production and had increased levels of mtDNA damage compared with those in haplogroup H. Differences in bioenergetic capacity were also observed in that human umbilical vein endothelial cells belonging to haplogroup L had decreased maximal bioenergetic capacities compared with haplogroup H. Analysis of peripheral blood mononuclear cells from age-matched healthy controls with West Eurasian or African maternal ancestries showed that haplogroups sharing an A to G mtDNA mutation at nucleotide pair 10398 had increased mtDNA ...Continue Reading

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Citations

Feb 9, 2017·Current Opinion in Cardiology·Alexander W Bray, Scott W Ballinger
Apr 20, 2018·Nature Reviews. Rheumatology·Francisco J BlancoIgnacio Rego-Pérez
Dec 14, 2018·Cancer Metastasis Reviews·Thomas C BeadnellDanny R Welch
May 12, 2019·GeroScience·Gabriela E Farias QuipildorDerek M Huffman
May 10, 2017·American Journal of Respiratory and Critical Care Medicine·Jegen KandasamyNamasivayam Ambalavanan
Jun 9, 2020·Redox Biology·Jamelle A BrownScott W Ballinger
Oct 11, 2017·Mitochondrion·Jessica L FettermanDaniel Levy
Mar 20, 2021·Pulmonary Circulation·Andrew J BryantLei Jin

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