Endothelial cell density in porcine corneas after exposure to hypotonic solutions.

Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie
Christian MeltendorfJan Schroeter

Abstract

To evaluate exposure to sucrose solution (1.8%) and hypotonic balanced salt solution (BSS) for its effects on endothelial cell density of porcine corneas. Two groups of central discs from pig corneas were organ-cultured for 24 h. Twelve corneas per group were exposed to sucrose solution (1.8%) or hypotonic BSS for 4 min each. The paired corneal discs were not treated and served as controls. After further organ culture with and without dextran for 48 h, corneal endothelium was stained with alizarin red and examined by light microscopy. The endothelial cell densities were determined manually on three central images. The endothelial cell density differed significantly between corneas exposed to sucrose and the control corneas (3982+/-382 cells/mm(2) and 4360+/-331 cells/mm(2) respectively, and 3876+/-364 cells/mm(2) versus 4374+/-168 cells/mm(2) respectively with 6% dextran). In contrast, the endothelial cell density did not differ significantly between corneas exposed to hypotonic BSS and the control corneas (4374+/-296 cells/mm(2) and 4317+/-193 cells/mm(2) respectively, and 4348+/-151 cells/mm(2) versus 4426+/-175 cells/mm(2), respectively with 6% dextran). Exposure to 1.8% sucrose for 4 min induces a significant endothelial ce...Continue Reading

References

Apr 1, 1976·American Journal of Ophthalmology·H F EdelhauserR A Hyndiuk
Oct 1, 1988·Cryobiology·W J ArmitageD L Easty
Jan 1, 1986·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·S Sperling
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Sep 1, 1993·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·M Hagenah, M Böhnke
Feb 20, 1998·Acta Ophthalmologica Scandinavica·J Maas-ReijsA B Tullo

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Citations

Oct 29, 2017·Cell and Tissue Banking·Berenike C KunzmannMaximilian Schultheiss
Nov 21, 2007·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Jan SchroeterPeter Rieck
Feb 28, 2019·Cell and Tissue Banking·Daniel A WenzelMaximilian Schultheiss
Mar 21, 2019·Current Eye Research·Daniel A WenzelMaximilian Schultheiss
Jan 13, 2015·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Jan SchroeterChristian Meltendorf
Mar 8, 2020·Scientific Reports·Eric J SniderDavid O Zamora

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