Endothelial cell dynamics during anastomosis in vitro

Integrative Biology : Quantitative Biosciences From Nano to Macro
Anthony Diaz-SantanaAbraham D Stroock

Abstract

Vascular anastomosis - the fusion of vessels from two distinct branches of the vascular system - represents a critical step in vascular growth under both healthy and pathological conditions, in vivo, and presents an important target for engineering of vascularized tissues, in vitro. Recent works in animal models have advanced our understanding of the molecular and cellular players in vascular anastomosis, but questions remain related to cellular dynamics and control of this process, in vitro. In this study, we exploited a three-dimensional (3-D) culture platform to examine the dynamics of endothelial cell (EC) during and after vascular anastomosis by allowing angiogenesis and vasculogenesis to proceed in parallel. We show that anastomosis occurs between sprouts formed by angiogenesis from an endothelium and tubes formed by vasculogenesis in the bulk of a 3-D matrix. This fusion leads to highly connected vessels that span from the surface of the matrix into the bulk in a manner that depends on cell density and identity. Further, we observe and analyze intermixing of endothelial cells of distinct origin (surface versus bulk) within the vessels structures that are formed; we provide evidence that the cells migrate along pre-existi...Continue Reading

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Citations

Jan 14, 2016·Integrative Biology : Quantitative Biosciences From Nano to Macro·Young Hye SongClaudia Fischbach
Mar 26, 2016·Frontiers in Physiology·Myra N ChávezJosé T Egaña
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Mar 9, 2019·Microbiology Spectrum·Dorian Obino, Guillaume Duménil

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