Endothelial cell heparanase modulation of lipoprotein lipase activity. Evidence that heparan sulfate oligosaccharide is an extracellular chaperone.

The Journal of Biological Chemistry
S PillarisettiI J Goldberg

Abstract

A unique feature of lipoprotein lipase (LpL), the rate-limiting enzyme in the hydrolysis of circulating triglycerides, is its movement from its cell of synthesis, adipocyte or myocyte, to its site of action, the luminal endothelial surface. This involves processes that allow LpL to be released from the adipocyte cell surface and transferred against the flow of interstitial fluid to the luminal surface of endothelial cells. LpL, an unstable enzyme, must retain its activity during this process. Whether a chaperone-like molecule is involved in LpL stabilization and transport is unclear. In the present study, we tested the hypothesis that endothelial cells secrete factors that release LpL and promote its transfer to the luminal endothelial surface. Incubation of adipocytes with endothelial cell conditioned medium (ECCM) led to release of about 2-fold more LpL activity than control medium. Medium from endothelial cells exposed to lysophosphatidylcholine (lyso-ECCM), a product of LpL lipolysis of lipoproteins, released approximately 3-fold more LpL than ECCM. Concomitant with the release of LpL, adipocyte cell surface heparan sulfate (HS) proteoglycans were degraded suggesting that lyso-ECCM contained a heparanase-like activity. More...Continue Reading

References

Oct 1, 1992·Current Opinion in Cell Biology·T N WightE E Qwarnström
Nov 1, 1992·The Journal of Clinical Investigation·K TavangarF B Kraemer
Jan 1, 1992·Advances in Experimental Medicine and Biology·D D CunninghamD H Farrell
Dec 1, 1992·Arteriosclerosis and Thrombosis : a Journal of Vascular Biology·M F StinsI J Goldberg
Jan 1, 1991·Annual Review of Biochemistry·L Kjellén, U Lindahl
Jan 1, 1991·Methods in Enzymology·G Bengtsson-Olivecrona, T Olivecrona
May 1, 1990·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·U SaxenaI J Goldberg
Apr 1, 1990·Biochemical Society Transactions·J T GallagherM Lyon
Apr 1, 1990·The American Journal of Physiology·L CampsT Olivecrona
Apr 20, 1989·The New England Journal of Medicine·R H Eckel
Oct 1, 1987·Seminars in Thrombosis and Hemostasis·J A Marcum, R D Rosenberg
May 16, 1988·Biochemical and Biophysical Research Communications·M M Maimone, D M Tollefsen
May 1, 1984·Atherosclerosis·L JonassonT Olivecrona
Jan 1, 1993·Annual Review of Biochemistry·J P Hendrick, F U Hartl
Aug 1, 1993·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·G David
Jun 21, 1996·The Journal of Biological Chemistry·P SivaramI J Goldberg
Jan 1, 1995·Annual Review of Cell and Developmental Biology·R M NelsonI Stamenkovic

❮ Previous
Next ❯

Citations

Mar 15, 2000·The International Journal of Biochemistry & Cell Biology·S TumovaJ R Couchman
Sep 17, 2014·Molecular and Cellular Endocrinology·Aikaterini TheodorakiPierre-Marc Bouloux
Feb 7, 2014·Canadian Journal of Diabetes·Fang WangBrian Rodrigues
Oct 26, 2011·Biochimica Et Biophysica Acta·Min Suk KimBrian Rodrigues
Jun 26, 2003·Cancer Science·Hitoshi KurokawaMasaki Yanagishita
Dec 3, 2014·Atherosclerosis·Yuan LiChao-Ke Tang
Dec 3, 2014·Biochimica Et Biophysica Acta·Ying Wang, Brian Rodrigues
Oct 19, 2011·Acta Biomaterialia·Juthamas Ratanavaraporn, Yasuhiko Tabata
Nov 4, 2004·Journal of Molecular and Cellular Cardiology·Thomas PulinilkunnilBrian Rodrigues
Jul 13, 2005·Developmental Biology·Eleanor ChenStephen C Ekker
Aug 3, 2004·Laboratory Investigation; a Journal of Technical Methods and Pathology·Takaomi OhkawaNoriaki Tanaka
Feb 14, 2009·Circulation Research·Christian Aalkjaer, Donna Briggs Boedtkjer
Jan 15, 2000·Arteriosclerosis, Thrombosis, and Vascular Biology·J C ObunikeI J Goldberg
Oct 16, 1999·Arteriosclerosis, Thrombosis, and Vascular Biology·G PlenzH Robenek
Mar 9, 2013·Arteriosclerosis, Thrombosis, and Vascular Biology·Ying WangBrian Rodrigues
Nov 26, 2011·Arteriosclerosis, Thrombosis, and Vascular Biology·Fang WangBrian Rodrigues
Feb 3, 2005·American Journal of Physiology. Endocrinology and Metabolism·Ding AnBrian Rodrigues
Mar 15, 2019·Protein Science : a Publication of the Protein Society·Samantha PiszkiewiczGary J Pielak
Mar 7, 2006·The Journal of Biological Chemistry·Shallee PageAndré Bensadoun
Jun 6, 2006·American Journal of Physiology. Heart and Circulatory Physiology·Ding An, Brian Rodrigues
May 8, 2008·American Journal of Physiology. Endocrinology and Metabolism·Girish KewalramaniBrian Rodrigues
Feb 17, 2009·American Journal of Physiology. Heart and Circulatory Physiology·Fang WangBrian Rodrigues
Feb 1, 2005·American Journal of Physiology. Heart and Circulatory Physiology·Thomas PulinilkunnilBrian Rodrigues
Jul 21, 1998·The Journal of Biological Chemistry·D S PikasU Lindahl
Dec 22, 1999·The Journal of Biological Chemistry·M Bogdanov, W Dowhan
Aug 8, 1998·The Journal of Biological Chemistry·N ParthasarathyB Mulloy
Jun 28, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·M ElkinI Vlodavsky
Apr 17, 2014·American Journal of Physiology. Endocrinology and Metabolism·Amy Pei-Ling ChiuBrian Rodrigues
Aug 14, 1999·The Journal of Biological Chemistry·M Toyoshima, M Nakajima
Nov 25, 2003·The Journal of Biological Chemistry·Jane ReilandDario Marchetti
Jun 20, 2006·The Journal of Biological Chemistry·Dorothe SpillmannGunilla Olivecrona
Apr 26, 2020·Proceedings of the National Academy of Sciences of the United States of America·Kathryn H GunnSaskia B Neher

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

ApoE, Lipids & Cholesterol

Serum cholesterol, triglycerides, apolipoprotein B (APOB)-containing lipoproteins (very low-density lipoprotein (VLDL), immediate-density lipoprotein (IDL), and low-density lipoprotein (LDL), lipoprotein A (LPA)) and the total cholesterol/high-density lipoprotein (HDL) cholesterol ratio are all connected in diseases. Here is the latest research.