Endothelial cell monolayer dysfunction caused by oxidized low density lipoprotein: attenuation by oleic acid

Prostaglandins, Leukotrienes, and Essential Fatty Acids
R J KarmanC M Hart

Abstract

Oleic acid (18:1) may exert beneficial effects on the pathogenesis of vascular disease by a variety of mechanisms. To determine if 18:1 exerts direct protective effects on vascular endothelial cells, porcine pulmonary artery endothelial cells (PAEC) were supplemented with 0.1 mM 18:1, gamma-linolenic acid (18:3), or ethanol vehicle (ETOH) prior to treatment with low density lipoprotein (LDL), or CU(2+)-oxidized LDL (OXLDL). Treatment with neither LDL nor OXLDL (100 micrograms protein/ml) for 24-48 h caused PAEC cytotoxicity, whereas OXLDL, but not LDL, caused derangements in PAEC actin microfilament architecture and monolayer barrier dysfunction. Supplementation with 18:1, but not 18:3, attenuated derangements caused by OXLDL and lysophosphatidylcholine, a component of OXLDL. These results demonstrate that monounsaturated fatty acids directly alter the response of vascular endothelial cells to OXLDL and may retard the atherosclerotic process by decreasing the efflux of macromolecules (e.g. LDL) into the vessel wall.

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Citations

Mar 3, 1998·Prostaglandins, Leukotrienes, and Essential Fatty Acids·C M HartE R Mohler
Jul 18, 2002·Critical Reviews in Clinical Laboratory Sciences·Minna L HannukselaMarkku J Savolainen
Sep 17, 2002·Arteriosclerosis, Thrombosis, and Vascular Biology·Cristina RodríguezLina Badimon
Apr 29, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Kwan-Sik KimYan Xu
Sep 18, 1998·Current Opinion in Lipidology·S Tsimikas, P D Reaven
Jan 5, 2002·The American Journal of Clinical Nutrition·Michal ToborekBernhard Hennig

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