Endothelial Cell Morphology Regulates Inflammatory Cells Through MicroRNA Transferred by Extracellular Vesicles

Frontiers in Bioengineering and Biotechnology
Jiaqi LiangYue Zhou

Abstract

Vascular inflammation plays an important role in the pathogenesis and the development of cardiovascular diseases such as arteriosclerosis and restenosis, and the dysfunction of endothelial cells (ECs) may result in the activation of monocytes and other inflammatory cells. ECs exhibit an elongated morphology in the straight part of arteries but a cobblestone shape near the pro-atherogenic region such as branch bifurcation. Although the effects of hemodynamic forces on ECs have been widely studied, it is not clear whether the EC morphology affects its own function and thus the inflammatory response of monocytes. Here we showed that elongated ECs cultured on poly-(dimethyl siloxane) membrane surface with microgrooves significantly suppressed the activation of the monocytes in co-culture, in comparison to ECs with a cobblestone shape. The transfer of EC-conditioned medium to monocytes had the same effect, suggesting that soluble factors were involved in EC-monocyte communication. Further investigation demonstrated that elongated ECs upregulated the expression of anti-inflammatory microRNAs, especially miR-10a. Moreover, miR-10a was found in the extracellular vesicles (EVs) released by ECs and transferred to monocytes, and the inhib...Continue Reading

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Citations

May 1, 2021·International Journal of Molecular Sciences·Allison MathiesenAnca Dobrian
Jan 23, 2022·Molecular and Cellular Biochemistry·Shruti Sharma, Ujjawal Sharma
Sep 9, 2021·Inflammation·Marina FandarosWei Yin

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Methods Mentioned

BETA
scanning electron microscopy
PCR
enzyme-linked immunosorbent assay
Transfection
electrophoresis
ELISA
transmission electron microscopy

Software Mentioned

GraphPad Prism
Zeta View

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