Endothelial cell oxidant generation during K(+)-induced membrane depolarization

Journal of Cellular Physiology
A B Al-MehdiA B Fisher

Abstract

We tested the hypothesis that membrane depolarization may initiate oxidant generation in the endothelial cell. Depolarization was produced in bovine pulmonary arterial endothelial cells (BPAEC) in monolayer culture with varying external K+, or with glyburide (10 microM), tetraethylammonium (TEA, 10 mM), gramicidin (1 microM), or nigericin (2 microM). Evaluation of bisoxonol fluorescence of BPAEC indicated concentration-dependent depolarization by high K+ (2% change in fluorescence/mV change in membrane potential in the 5.9-48 mM range of K+) and essentially complete depolarization with glyburide. Generation of oxidants was assessed with o-phenylenediamine dihydrochloride (o-PD) oxidation in the presence of horseradish peroxidase (HRP). There was a time-dependent increase in o-PD oxidation with 24 mM K+, nigericin, and gramicidin over 2 hours compared with control. In 1 hour o-PD oxidation increased 2.8-fold for 24 mM and 3.7-fold for 48 mM K+ compared with control. Catalase reduced 24 mM K(+)-induced o-PD oxidation by 50%, while Cu/Zn-superoxide dismutase (SOD) abolished the increase. Oxidation of o-PD was reduced by 57% in the absence of HRP in the system. With K+ channel blockade, o-PD oxidation increased 3.8-fold with glybur...Continue Reading

References

Feb 1, 1977·Journal of Cellular Physiology·R SmallA B Fisher
Nov 1, 1992·Archives of Biochemistry and Biophysics·H IschiropoulosJ S Beckman
Feb 1, 1992·American Journal of Respiratory Cell and Molecular Biology·V L KinnulaJ D Crapo
Mar 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·R E LaskeyC van Breemen
Apr 1, 1992·Journal of Applied Physiology·R G EckenhoffA B Fisher
Jul 1, 1992·Archives of Biochemistry and Biophysics·I S AyeneA B Fisher
Dec 9, 1991·Biochimica Et Biophysica Acta·C Bronner, Y Landry
Aug 1, 1991·The Journal of Clinical Investigation·A B FisherR G Eckenhoff
Feb 1, 1991·Journal of Molecular and Cellular Cardiology·D L CampbellA R Whorton
Jun 1, 1990·The Journal of Infectious Diseases·R A Clark
Jul 29, 1988·Biochemical and Biophysical Research Communications·K R WhitmerA Schwartz
Oct 1, 1989·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·D J AdamsC Van Breemen
Jun 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J L ZweierG A Lutty
Jan 1, 1985·Annals of the New York Academy of Sciences·E A Jaffe
Jan 1, 1984·Annual Review of Pharmacology and Toxicology·R F Furchgott
Apr 11, 1984·Biochimica Et Biophysica Acta·T BräunerR J Strasser
Jan 1, 1994·Free Radical Biology & Medicine·S I Liochev, I Fridovich
Jan 1, 1993·Arthritis and Rheumatism·G F FalascaA J Reginato
Feb 1, 1993·Free Radical Biology & Medicine·P C PanusB A Freeman
Jan 29, 1993·Biochemical and Biophysical Research Communications·C M BrophyB E Sumpio

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Citations

Nov 5, 2008·Cell Biochemistry and Biophysics·Shampa ChatterjeeAron B Fisher
Dec 2, 2000·European Journal of Pharmacology·V E MendizabalE Adler-Graschinsky
Jan 1, 1997·Free Radical Biology & Medicine·A B al-MehdiA B Fisher
Oct 18, 2011·American Journal of Physiology. Heart and Circulatory Physiology·Shampa ChatterjeeAron B Fisher
Jun 24, 2008·American Journal of Physiology. Lung Cellular and Molecular Physiology·Thomas H ThatcherPatricia J Sime
Nov 14, 1997·American Journal of Respiratory and Critical Care Medicine·E N AtochinaA B Fisher
Nov 14, 1997·American Journal of Respiratory and Critical Care Medicine·F SakamakiF W Schildberg
Aug 3, 1999·American Journal of Respiratory and Critical Care Medicine·O D SchneuwlyD R Morel
Jun 6, 1998·American Journal of Respiratory Cell and Molecular Biology·A B Al-MehdiA B Fisher
Oct 3, 2002·Hypertension Research : Official Journal of the Japanese Society of Hypertension·Tsuneaki SadanagaIsao Abe
Sep 2, 1997·Proceedings of the National Academy of Sciences of the United States of America·M A CiorbaT Hoshi
Nov 15, 2011·Annual Review of Physiology·Elizabeth A BrowningAron B Fisher
Aug 12, 2015·Current Opinion in Organ Transplantation·Mary K PorteousJason D Christie
Nov 12, 2005·Integrative Cancer Therapies·Mark F McCarty, Keith I Block
May 11, 2002·Critical Care Medicine·Aron B FisherYefim Manevich
Aug 28, 2004·American Journal of Physiology. Heart and Circulatory Physiology·Ikuo MatsuzakiAron B Fisher
Aug 10, 2013·Clinical Journal of the American Society of Nephrology : CJASN·Kristen L JablonskiDouglas R Seals
Apr 12, 2015·American Journal of Physiology. Heart and Circulatory Physiology·Shampa ChatterjeeAron B Fisher
Oct 1, 1998·The American Journal of Physiology·E N AtochinaV R Muzykantov
Sep 23, 2014·American Journal of Physiology. Lung Cellular and Molecular Physiology·Shampa ChatterjeeAron B Fisher
Sep 27, 2001·Endothelium : Journal of Endothelial Cell Research·C E Patterson, H Lum
Aug 19, 2005·American Journal of Physiology. Cell Physiology·Tatyana MilovanovaAron B Fisher
May 25, 2021·Seminars in Respiratory and Critical Care Medicine·Jake G Natalini, Joshua M Diamond

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