Endothelial cell response to hypoxia-reoxygenation is mediated by IL-1

The Journal of Surgical Research
E T ClarkB L Gewertz

Abstract

Reperfusion injury involves the adhesion and activation of neutrophils (PMN) both in affected tissues and distant organs. Cell adhesion molecules (CAM) such as endothelial-leukocyte adhesion molecule-1 (ELAM-1) and intercellular adhesion molecule-1 (ICAM-1) are known to mediate, at least in part, the adherence of activated PMN to the endothelium. To characterize the cellular mechanisms of this phenomenon, we exposed cultured human umbilical vein endothelial cells (HU-VEC) to hypoxia and reoxygenation (H/R) using an incubator chamber purged of oxygen with 100% nitrogen. Confluent monolayers of HUVEC were subjected to 60 min of hypoxia followed by variable periods of reoxygenation (120 and 240 min). Flow cytometry was utilized to assess the expression of ELAM-1 and ICAM-1, expressed as percent shift from baseline expression. To determine what role endothelium-derived cytokines such as IL-1 play in the expression of CAM after H/R, we performed additional experiments in the presence of recombinant IL-1 receptor antagonist (IL-1RA). ICAM-1 was present on unstimulated HUVEC while ELAM-1 was not constitutively expressed. Following exposure of cells to hypoxia and reoxygenation, significant increases in ELAM expression were seen (8.4 +...Continue Reading

Citations

Jul 9, 1996·Proceedings of the National Academy of Sciences of the United States of America·G ZündS P Colgan
Aug 12, 1998·American Journal of Respiratory and Critical Care Medicine·N BaudryE Vicaut
Dec 1, 2001·American Journal of Respiratory Cell and Molecular Biology·B Beck-SchimmerP A Ward
Jun 11, 1999·The American Journal of Physiology·C WillamK U Eckardt
Apr 23, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Danielle G SouzaMauro M Teixeira
Oct 12, 1999·The American Journal of Physiology·A L Cooper, D Beasley
Sep 15, 1998·The Journal of Surgical Research·K NishidaK Okinaga

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