Abstract
In this study, endothelial cell (EC)-seeded nanocomposite grafts were preconditioned with 1-2 dynes/cm(2) in vitro to establish whether low shear stress resulted in improved cell adherence prior to physiological shear stress (15 dynes/cm(2)). Alamar blue cell viability was assessed. Polymerase chain reaction was conducted for glyceraldehyde-3-phosphate dehydrogenase, transforming growth factor beta-1 (TGFbeta-1), vascular endothelial growth factor receptor-1 (VEGFR-1), platelet EC adhesion molecule-1, and vascular endothelial growth factor receptor-2 (VEGFR-2). The Alamar blue results demonstrated improved cellular retention following preconditioning (P < 0.001). VEGFR-2 and TGFbeta-1 expression was up-regulated, and VEGFR-1 down-regulated following preconditioning. This investigation confirms previous findings regarding the potential benefits of preconditioning, and demonstrates that these benefits can be applied to ECs seeded on the nanocomposite employed. It also demonstrates further the suitability and potential of nanocomposite for future use in tissue-engineered cardiovascular devices.
References
May 1, 1995·The Journal of Cell Biology·T Mustonen, K Alitalo
Jan 1, 1995·Blood Purification·B J Ballermann, M J Ott
Mar 1, 1995·Surgery·M J Ott, B J Ballermann
Aug 1, 1997·Arteriosclerosis, Thrombosis, and Vascular Biology·H UebaT Yaginuma
Sep 15, 1998·Kidney International. Supplement·B J BallermannA Liu
Jan 12, 1999·Journal of Vascular Surgery·A DardikB J Ballermann
May 16, 2000·Biomaterials·J Y ZhangS Agarwal
Apr 5, 2001·Journal of Biomedical Materials Research·A M SeifalianG Hamilton
Jun 25, 2002·Proceedings of the National Academy of Sciences of the United States of America·Ayelet Shay-SalitNitzan Resnick
Mar 23, 2004·Biotechnology and Applied Biochemistry·Mohamed BaguneidAlexander M Seifalian
May 4, 2004·European Journal of Vascular and Endovascular Surgery : the Official Journal of the European Society for Vascular Surgery·S T RashidA M Seifalian
May 13, 2005·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Ruben Y KannanAlexander M Seifalian
May 26, 2005·Biomaterials·Geoffrey PunshonAlexander M Seifalian
Oct 29, 2005·Biomaterials·Ruben Y KannanAlexander M Seifalian
Jun 8, 2006·Biotechnology and Applied Biochemistry·Dina S VaraAlexander M Seifalian
Citations
Feb 28, 2009·Artificial Organs·Paul S Malchesky
May 19, 2011·International Journal of Nanomedicine·Hossein GhanbariAlexander M Seifalian
Dec 18, 2013·Antioxidants & Redox Signaling·Shampa Chatterjee, Aron B Fisher
Mar 13, 2014·Tissue Engineering. Part a·Jaroslav ChlupacLaurence Bordenave
Jul 16, 2016·Artificial Organs·Nasim Salehi-NikJenneke Klein-Nulend
Oct 5, 2010·Cell Transplantation·Huey-Shan HungShinn-Zong Lin
Dec 20, 2018·Journal of Cellular Physiology·Thaís Silva PintoWillian F Zambuzzi