PMID: 2122822Dec 1, 1990Paper

Endothelial cell toxicity of solid-organ preservation solutions

The Annals of Thoracic Surgery
U O von OppellB Reichart

Abstract

Endothelial cell damage caused by myocardial cardioplegic solutions (Bretschneider HTK and St. Thomas' Hospital No. 2) or renal and hepatic cold storage solutions (modified Collins and University of Wisconsin solution) was assessed in monolayer cultures of adult human venous endothelial cells at 4 degrees to 10 degrees C with phase-contrast microscopy. St. Thomas' Hospital solution caused the cells to contract, resulting in disruption of monolayer integrity and opening of intercellular gaps, and resulted in a 24-hour postexposure survival of 51.0% +/- 2.4%. Bretschneider HTK solution altered cellular morphology less and produced the best postexposure survival (80.2% +/- 2.6%; p less than 0.001). Although morphology was altered the least with University of Wisconsin solution, postexposure survival with this solution, which was similar to that with modified Collins solution, was superior to that with St. Thomas' (p less than 0.01) but inferior to that with Bretschneider HTK (p less than 0.05). The superior protection provided by Bretschneider HTK was due to its additives histidine, tryptophan, and KH-2-oxygluterate (p less than 0.005), and to its low chloride content (p less than 0.005). Furthermore, modifying St. Thomas' solutio...Continue Reading

References

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Citations

Feb 1, 1996·The Annals of Thoracic Surgery·N IsowaH Wada
Aug 1, 1996·The Annals of Thoracic Surgery·T EberlR Margreiter
May 23, 2002·The Annals of Thoracic Surgery·Francesco AlamanniValeria Dall'Asta
Jan 22, 1998·The Annals of Thoracic Surgery·A ParolariP Biglioli
May 18, 1999·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·E KevelaitisP Menasché
Jul 19, 2002·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Desley A H NeilDavid J Effeney
Apr 11, 2003·Fundamental & Clinical Pharmacology·S T L AbrahamseT M van Gulik
Jun 6, 2012·European Journal of Cardio-thoracic Surgery : Official Journal of the European Association for Cardio-thoracic Surgery·Fabiano F VianaGeorge Matalanis
Jan 29, 2011·Regional Anesthesia and Pain Medicine·Christof BirkenmaierSusanne Mayer-Wagner
Jan 1, 1993·Journal of Cardiac Surgery·P ZillaM Deutsch
Mar 1, 1993·The Annals of Thoracic Surgery·R CartierL C Pelletier
Apr 1, 1996·European Journal of Vascular and Endovascular Surgery : the Official Journal of the European Society for Vascular Surgery·K P WalluscheckA Haverich
Feb 1, 1994·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·C A CosenzaL Makowka
Nov 14, 1997·Artificial Cells, Blood Substitutes, and Immobilization Biotechnology·M Mathy-HartertJ G Riess
Jul 1, 1996·Journal of Cardiovascular Pharmacology·J G GrohsG Raberger
May 1, 1995·The Journal of International Medical Research·P CarbognaniP Bobbio
Aug 1, 1994·The Annals of Thoracic Surgery·T EberlR Margreiter

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